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Development and validation of a high‐performance liquid chromatography method for the quantification of talazoparib in rat plasma: Application to plasma protein binding studies

Published: Aug 15, 2017

Author(s): Mahendra Kumar Hidau, Srikanth Kolluru, Srinath Palakurthi

Journal: Biomedical Chromatography

Abstract A sensitive and selective RP‐HPLC method has been developed and validated for the quantification of a highly potent poly ADP ribose polymerase inhibitor talazoparib (TZP) in rat plasma. Chromatographic separation was performed with isocratic elution method. Absorbance for TZP was measured with a UV detector (SPD‐20A UV–vis) at a λmax of 227 nm. Protein precipitation was used to extract the drug from plasma samples using methanol–acetonitrile...

Read More thumbnail image: Development and validation of a highperformance liquid chromatography method for the quantification of talazoparib in rat plasma Application to plasma protein binding studies

Application of headspace solid‐phase microextraction followed by gas chromatography coupled with mass spectrometry to determine esters of carboxylic acids and other volatile compounds in Dermestes maculatus and Dermestes ater lipids

Published: Aug 15, 2017

Author(s): Magdalena Cerkowniak, Mieczysława I. Boguś, Emilia Włóka, Piotr Stepnowski, Marek Gołębiowski

Journal: Biomedical Chromatography

Abstract A constant problem in veterinary medicine, human healthcare, agriculture, forestry and horticulture is the large number of pests, and the lack of effective methods to combat them which cause no harm to the rest of the environment. It is recommended and desired to reduce the use of chemicals and increase the use of agents based on knowledge acquired in the fields of biology, chemistry and agrochemicals. To learn the defense...

Read More thumbnail image: Application of headspace solidphase microextraction followed by gas chromatography coupled with mass spectrometry to determine esters of carboxylic acids and other volatile compounds in Dermestes maculatus and Dermestes ater lipids

Chromatographic HPV‐16 E6/E7 plasmid vaccine purification employing L‐histidine and 1‐benzyl‐L‐histidine affinity ligands

Published: Aug 14, 2017

Author(s): Lúcia F. A. Amorim, Rita Gaspar, Patrícia Pereira, Urh Černigoj, Fani Sousa, João António Queiroz, Ângela Sousa

Journal: ELECTROPHORESIS

Affinity chromatography based on amino acids as interacting ligands was already indicated as an alternative compared to ion exchange or hydrophobic interaction for plasmid DNA purification. Understanding the recognition mechanisms occurring between histidine‐based ligands and nucleic acids enables more efficient purification of a DNA vaccine, as the binding and elution conditions can be adjusted in order to enhance the purification...

Read More thumbnail image: Chromatographic HPV16 E6E7 plasmid vaccine purification employing Lhistidine and 1benzylLhistidine affinity ligands

Silver nanoparticles functionalized with ampicillin

Published: Aug 14, 2017

Author(s): Agnieszka Rogowska, Katarzyna Rafińska, Paweł Pomastowski, Justyna Walczak, Viorica Railean‐Plugaru, Magdalena Buszewska‐Forajta, Bogusław Buszewski

Journal: ELECTROPHORESIS

The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high‐performance liquid...

Read More thumbnail image: Silver nanoparticles functionalized with ampicillin

Checking transfer efficiency and equal loading via qualitative optical way in western blotting

Published: Aug 14, 2017

Author(s): Jun‐Hua Gong, Jian‐Ping Gong, Kai‐Wen Zheng

Journal: ELECTROPHORESIS

The ability to determine that successful transfer and equal loading occur prior to using primary antibodies is important. And total protein staining is commonly used to check transfer efficiency and normalization, which play a crucial role in western blotting. Ponceau S and coomassie blue are commonly used, but there are disadvantages reported in recent years. Therefore, we are interested in finding another method, which is cheap, easy...

Read More thumbnail image: Checking transfer efficiency and equal loading via qualitative optical way in western blotting

An LC–MS/MS method for quantitation of cyanidin‐3 ‐O‐ glucoside in rat plasma: Application to a comparative pharmacokinetic study in normal and streptozotocin‐induced diabetic rats

Published: Aug 13, 2017

Author(s): Chunxia Yang, Qiuhua Wang, Shenbao Yang, Qiong Yang, Ying Wei

Journal: Biomedical Chromatography

Abstract A sensitive and reliable liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed to determine cyanidin‐3‐O‐glucoside (Cy‐3G) in normal and streptozotocin‐induced diabetic rat plasma. Chromatographic separation was carried out on a Zorbax SB‐C18 (50 × 4.6 mm, 5 μm) column and mass spectrometric analysis was performed using a Thermo Finnigan TSQ Quantum Ultra triple‐quadrupole mass spectrometer coupled with an ESI...

Read More thumbnail image: An LCMSMS method for quantitation of cyanidin3 O glucoside in rat plasma Application to a comparative pharmacokinetic study in normal and streptozotocininduced diabetic rats

Quality control in mass spectrometry‐based proteomics

Published: Aug 12, 2017

Author(s): Wout Bittremieux, David L. Tabb, Francis Impens, An Staes, Evy Timmerman, Lennart Martens, Kris Laukens

Journal: Mass Spectrometry Reviews

Mass spectrometry is a highly complex analytical technique and mass spectrometry‐based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of...

Read More thumbnail image: Quality control in mass spectrometrybased proteomics

Quality control in mass spectrometry‐based proteomics

Published: Aug 12, 2017

Author(s): Wout Bittremieux, David L. Tabb, Francis Impens, An Staes, Evy Timmerman, Lennart Martens, Kris Laukens

Journal: Mass Spectrometry Reviews

Mass spectrometry is a highly complex analytical technique and mass spectrometry‐based proteomics experiments can be subject to a large variability, which forms an obstacle to obtaining accurate and reproducible results. Therefore, a comprehensive and systematic approach to quality control is an essential requirement to inspire confidence in the generated results. A typical mass spectrometry experiment consists of...

Read More thumbnail image: Quality control in mass spectrometrybased proteomics

Structural characterization and pharmacological evaluation of the new synthetic cannabinoid CUMYL‐PEGACLONE

Published: Aug 9, 2017

Author(s): Verena Angerer, Lukas Mogler, Jan‐Patrick Steitz, Philippe Bisel, Cornelius Hess, Clara T. Schoeder, Christa E. Müller, Laura M. Huppertz, Folker Westphal, Jan Schäper, Volker Auwärter

Journal: Drug Testing and Analysis

The number of new psychoactive substances (NPS) that have emerged on the European market has been rapidly growing in recent years, with a particularly high number of new compounds from the group of synthetic cannabinoid receptor agonists. There have been various political efforts to control the trade and the use of NPS worldwide. In Germany, the Act to control the distribution of new psychoactive substances (NpSG) came into force in November 2016. In...

Read More thumbnail image: Structural characterization and pharmacological evaluation of the new synthetic cannabinoid CUMYLPEGACLONE

Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion

Published: Aug 9, 2017

Author(s): Olivier Salamin, Jonathan Mignot, Tiia Kuuranne, Martial Saugy, Nicolas Leuenberger

Journal: Drug Testing and Analysis

Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymerase chain reaction as an alternative quantitative method, the present study supports that delta‐aminolevulinate synthase 2...

Read More thumbnail image: Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion

Pharmacokinetics and tissue distribution study of clevidipine and its primary metabolite H152/81 in rats

Published: Aug 8, 2017

Author(s): Yan Wang, Lanting Zhao, Tengfei Li, Wen Yang, Qian Li, Luning Sun, Li Ding

Journal: Biomedical Chromatography

Abstract This present study was designed to investigate the pharmacokinetic profiles and tissue distribution characteristics of clevidipine and its primary metabolite H152/81 in rats following a single intravenous administration of clevidipine butyrate injectable emulsion. For this study, a sensitive and selective liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was established and validated for the simultaneous...

Read More thumbnail image: Pharmacokinetics and tissue distribution study of clevidipine and its primary metabolite H15281 in rats

In situ chemical and morphological characterization of copper under near ambient reduction and oxidation conditions

Published: Aug 8, 2017

Author(s): H. Sezen, M. Al‐Hada, M. Amati, L. Gregoratti

Journal: Surface and Interface Analysis

By using spatially resolved photoelectron spectromicroscopy, we have investigated the formation of Cu(I) oxide phases when a bulk Cu foil is exposed to a near ambient pressure of molecular oxygen. The experiment was performed by using a novel cell for in operando measurements capable to overcome the so called ‘pressure gap’. We have found that the oxidation of Cu proceeds through the formation of Cu(I) oxide domains in the micron and...

Read More thumbnail image: In situ chemical and morphological characterization of copper under near ambient reduction and oxidation conditions

Preparation of large‐scale, durable, superhydrophobic PTFE films using rough glass templates

Published: Aug 8, 2017

Author(s): Yan Zhu, Yin He, Ji‐Fan Zhang, Zhen‐Hua Li, Wei Zhou, De‐Quan Yang, Edward Sacher

Journal: Surface and Interface Analysis

Large superhydrophobic polytetrafluoroethylene (PTFE) films, with good durability, were successfully prepared by a facile, low cost, environmentally friendly templating method, using a PTFE emulsion. For the first time, commercially available rough glass was employed as a reusable template. The results show that both the microstructure of the glass template and the concentration of the PTFE emulsion play important roles in the...

Read More thumbnail image: Preparation of largescale durable superhydrophobic PTFE films using rough glass templates

Simultaneous quantification of imatinib and its main metabolite N‐demethyl‐imatinib in human plasma by liquid chromatography–tandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor

Published: Aug 7, 2017

Author(s): Wei Zhuang, Hai‐Bo Qiu, Xin‐Meng Chen, Xiu‐Hong Yuan, Li‐Fang Yang, Xiao‐Wei Sun, Xiao‐Jun Zhou, Min Huang, Xue‐Ding Wang, Zhi‐Wei Zhou

Journal: Biomedical Chromatography

Abstract The aim of this study was to improve and validate a more stable and less time‐consuming method based on liquid chromatography and tandem mass spectrometry (LC‐ MS/MS) for the quantitative measurement of imatinib and its metabolite N‐demethyl‐imatinib (NDI) in human plasma. Separation of analytes was performed on a Waters XTerra RP18 column (50 × 2.1 mm i.d., 3.5 μm) with a mobile phase consisting of methanol–acetonitrile–water (65:20:15, v/v/v)...

Read More thumbnail image: Simultaneous quantification of imatinib and its main metabolite Ndemethylimatinib in human plasma by liquid chromatographytandem mass spectrometry and its application to therapeutic drug monitoring in patients with gastrointestinal stromal tumor

A UPLC‐TOF/MS‐based metabolomics study of rattan stems of Schisandra chinensis effects on Alzheimer's disease rats model

Published: Aug 7, 2017

Author(s): Bing‐You Yang, Jin‐Yan Tan, Yan Liu, Bo Liu, Shuang Jin, Hong‐Wei Guo, Hai‐Xue Kuang

Journal: Biomedical Chromatography

Abstract A UPLC‐TOF/MS‐based metabolomics method was established to explore the therapeutic mechanisms of rattan stems of S. chinensis (SCS) in Alzheimer's disease (AD). Experimental AD model was induced by intra‐hippocampal Aβ1–42 injection in rats. Cognitive function and oxidative stress condition in brain of AD rats were assessed using Morris water maze tests and antioxidant assays [malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione...

Read More thumbnail image: A UPLCTOFMSbased metabolomics study of rattan stems of Schisandra chinensis effects on Alzheimers disease rats model

Synchrotron radiation induced X‐ray production cross sections of 66Dy at energies across its Li ( i = 1–3 ) subshell absorption edges

Published: Aug 3, 2017

Author(s): Rajnish Kaur, Anil Kumar, M. Czyzycki, A. Migliori, A.G. Karydas, Sanjiv Puri

Journal: X-Ray Spectrometry

The X‐ray production (XRP) cross sections for the 66Dy Lk (k = l, α, η, β2,6,7,15, β1,6, β1,3,4,6, β2,7,15, γ1,5, γ2,3) emission lines have been measured by tuning the incident synchrotron radiation at energies over the range 7.8–9.2 keV and ~10–370 eV above the respective Li (i = 1–3) absorption edges. These measurements aim to check the reliability of the independent particle approximation models used to generate the...

Read More thumbnail image: Synchrotron radiation induced Xray production cross sections of 66Dy at energies across its Li  i13  subshell absorption edges

Study of gliquidone degradation behavior by high‐performance thin‐layer chromatography and ultra‐performance liquid chromatography methods

Published: Aug 3, 2017

Author(s): Nada S. Abdelwahab, Mohammed T. Elsaady, Aml G. Korany, Maha A. Hegazy

Journal: Biomedical Chromatography

Abstract Gliquidone (GQ) is an oral hypoglycemic agent, belonging to second‐generation sulfonylurea derivatives. New high‐performance thin‐layer chromatography (HPTLC) and ultra‐performance liquid chromatography (UPLC) methods have been developed and validated and used for complete stability study of GQ following International Conference on Harmonization guidelines. GQ was subjected to stress and forced degradation under hydrolytic,...

Read More thumbnail image: Study of gliquidone degradation behavior by highperformance thinlayer chromatography and ultraperformance liquid chromatography methods

A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electro‐chromatography with a methacrylate ester‐based monolithic column

Published: Aug 3, 2017

Author(s): Zhao Chen, Aili Xu, Xiaoli Bi, Wenhui Luo, Ji Li, Guorong Fan, Dongmei Sun

Journal: ELECTROPHORESIS

In the present study, a monolithic capillary column with higher permeability was developed for the in vivo discrimination of four coumarin analogs (bergapten, 2′‐acetylangelicin, imperatorin, and osthole) that typically require long separation times in HPLC. Instead of conventional methacrylate ester monolith (containing 19.5% porogen) with insufficient permeability (K = 1.52 – 1.66 × 10−14 M2) for plasma sample, the proposed...

Read More thumbnail image: A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electrochromatography with a methacrylate esterbased monolithic column

Sensing carbon allotropes in protective coatings on optical fibers with far‐ and near‐field R aman spectroscopy and microscopy

Published: Aug 2, 2017

Author(s): Sergey S. Kharintsev, Svetlana V. Saparina, Andrei A. Stolov, Jie Li, Alexandr I. Fishman

Journal: Journal of Raman Spectroscopy

Hermetic carbon coatings of different thicknesses ranging from 1 to 100 nm on a series of optical fibers are characterized with confocal Raman spectroscopy and atomic force microscopy. Standard far‐field Raman spectra in the region of 1,000–2,000 cm−1 were fitted and well reproduced using 5 individual components assigned to different species of graphite‐like and disordered phases. Based on the fitting, a new spectroscopic indicator was...

Read More thumbnail image: Sensing carbon allotropes in protective coatings on optical fibers with far and nearfield R aman spectroscopy and microscopy

Purity and adulteration in cocaine seizures and drug market inspection in Galicia (Spain) across an eight‐year period

Published: Aug 1, 2017

Author(s): Mª. de los Ángeles Villar Núñez, José Sánchez Morcillo, María Adolfina Ruíz Martínez

Journal: Drug Testing and Analysis

Read More thumbnail image: Purity and adulteration in cocaine seizures and drug market inspection in Galicia Spain across an eightyear period
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