EarlyView Articles
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Proteomics analysis of human pericardial fluid
Published: Aug 5, 2013
Author(s): Fei Xiang, Xuejiang Guo, Wen Chen, Jing Wang, Tao Zhou, Fuhua Huang, Changchun Cao, Xin Chen
Journal: PROTEOMICS
Pericardial fluid (PF) is considered as a biochemical window of heart. To date, there have been limited attempts to perform an in‐depth analysis of the PF proteome. In this study, an SDS‐PAGE‐LC‐MS/MS platform was utilized to explore depleted PF, which showed great coverage of low‐abundant proteins. In total, 1007 nonredundant proteins were identified with at least two peptides. This is the first comprehensive analysis of human PF...
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Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process
Published: Aug 5, 2013
Author(s): Qiying Yi, Ping Zhao, Xin Wang, Yong Zou, Xiaowu Zhong, Chen Wang, Zhonghuai Xiang, Qing‐you Xia
Journal: PROTEOMICS
The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid–crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the function of proteins in the silkworm ASG and the spinning mechanism. A total of 1132 proteins with 7647 unique peptides were identified in...
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Design and evaluation of capillary coupled with optical fiber light‐emitting diode induced fluorescence detection for capillary electrophoresis
Published: Aug 5, 2013
Author(s): Hongyun Ji, Meng Li, Lihong Guo, Hongyan Yuan, Chunling Wang, Dan Xiao
Journal: ELECTROPHORESIS
A new detector, capillary coupled with optical fiber LED‐induced fluorescence detector (CCOF‐LED‐IFD, using CCOF for short), is introduced for CE. The strategy of the present work was that the optical fiber and separation capillary were, in the parallel direction, fastened in a fixation capillary with larger inner diameter. By employing larger inner diameter, the fixation capillary allowed the large diameter of the optical fiber to be...
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LC‐MS/MS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study
Published: Aug 5, 2013
Author(s): Xiaolin Wang, Dan Zhang, Man Liu, Hongna Zhao, Aihua Du, Lingjie Meng, Huichen Liu
Journal: Biomedical Chromatography
ABSTRACT A sensitive and selective LC‐MS/MS method for the determination of agomelatine in human plasma was developed and validated. After simple liquid–liquid extraction, the analytes were separated on a Zorbax SB‐C18 column (150 × 2.1 mm i.d., 5 µm) with an isocratic mobile phase consisting of 5 mm ammonium acetate solution (containing 0.1% formic acid) and methanol (30:70, v/v) at a flow‐rate of 0.3 mL/min. The MS acquisition was performed...
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Low‐energy ion scattering: Determining overlayer thickness for functionalized gold nanoparticles
Published: Aug 5, 2013
Author(s): Ali Rafati, Rik Veen, David G. Castner
Journal: Surface and Interface Analysis
With the widespread use of engineered nanoparticles for biomedical applications, detailed surface characterization is essential for ensuring reproducibility and the quality/suitability of the surface chemistry to the task at hand. One important surface property to be quantified is the overlayer thickness of self‐assembled monolayer (SAM) functionalized nanoparticles, as this information provides insight into SAM ordering and assembly. We...
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Development and optimization of on‐line 2‐dimensional chromatographic approaches for eliminating matrix effects and improving bioanalysis of peptides in human plasma using UHPLC‐MS/MS
Published: Aug 5, 2013
Author(s): Omnia A. Ismaiel, Rand G. Jenkins
Journal: Drug Testing and Analysis
Online 2‐dimensional chromatographic approaches for eliminating matrix effects and optimizing bioanalysis of peptides using ultra‐high performance liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS) were studied. Three therapeutic peptides (octreotide, desmopressin, and vasopressin) were selected as model analytes. Human plasma was precipitated with acetonitrile; peptides were analyzed on C8, C18, Phenyl and HILIC ACQUITY UPLC...
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Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high‐speed counter‐current chromatography
Published: Aug 2, 2013
Author(s): Hui‐Lan Yue, Xiao‐Hui Zhao, Li‐Juan Mei, Yun Shao
Journal: Journal of Separation Science
Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high‐speed counter‐current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two‐phase solvent system composed of ethyl acetate/n‐butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27...
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Organic monoliths for high‐performance reversed‐phase liquid chromatography
Published: Aug 2, 2013
Author(s): Kun Liu, Pankaj Aggarwal, John S. Lawson, H. Dennis Tolley, Milton L. Lee
Journal: Journal of Separation Science
RPLC is the most common mode of LC. It is widely used for separations of both small and large molecules. Monolithic columns, which are currently under intensive study by many groups, have the potential of becoming attractive alternatives to particle‐packed columns. They are generally easier and faster to fabricate, and they demonstrate a lower pressure drop, less nonspecific adsorption, and richer chemistry (in the case of organic polymer...
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Ultra‐high performance size‐exclusion chromatography of synthetic polymers
Published: Aug 2, 2013
Author(s): Miroslav Janco, James N. Alexander, Edouard S. P. Bouvier, Damian Morrison
Journal: Journal of Separation Science
Ultra‐high performance size‐exclusion chromatography (UHP SEC) is a newly developed disruptive technology that allows the high‐resolution separation of synthetic polymers in as little as 2 min. The capability of UHP SEC for the characterization of synthetic polymers in organic solvents has been demonstrated. Using the Waters ACQUITY UPLC®1 H‐Class system and ethylene‐bridged hybrid size‐exclusion chromatography (SEC) columns packed with 1.7 to 2.5‐μm...
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Evaluation of the combinative application of SDS and sodium deoxycholate to the LC – MS ‐based shotgun analysis of membrane proteomes
Published: Aug 2, 2013
Author(s): Yong Lin, Kunbo Wang, Yujun Yan, Haiyan Lin, Bin Peng, Zhonghua Liu
Journal: Journal of Separation Science
SDS and sodium deoxycholate (SDC) as two representative detergents have been widely used in LC–MS/MS‐based shotgun analysis of membrane proteomes. However, some inherent disadvantages limit their applications such as interference with MS analysis or their weak ability to disrupt membranes. To address this, the combinative application of SDS and SDC was developed and evaluated in our study, which comprehensively used the strong ability of...
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Retention behavior of isomeric triacylglycerols in silver‐ion HPLC : E ffects of mobile phase composition and temperature
Published: Aug 2, 2013
Author(s): Miroslav Lísa, Rumen Denev, Michal Holčapek
Journal: Journal of Separation Science
A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver‐ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using the most widespread hexane‐ and dichloromethane‐based mobile phases. The randomization of mono‐acyl TG standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of TG isomers. The mobile phase composition has no influence on...
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Visualization and establishment of partial uniqueness and uniqueness rules in parallel factor analysis
Published: Aug 2, 2013
Author(s): Nematollah Omidikia, Hamid Abdollahi, Mohsen Kompany‐Zareh
Journal: Journal of Chemometrics
Parallel factor analysis (PARAFAC) is believed to be one of the most widely used multiway curve resolution techniques. The superiority of PARAFAC is due to its uniqueness property that is provided under mild assumptions. Unambiguously recovered chemically and physically meaningful profiles by PARAFAC are reliable for quantitative and qualitative application. The essential uniqueness condition of PARAFAC breaks totally or partially in data...
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Multivariate variographic versus bilinear data modeling
Published: Aug 2, 2013
Author(s): Pentti Minkkinen, Kim Harry Esbensen
Journal: Journal of Chemometrics
Two contrasting multivariate data sets (a process data series vs. a 1‐D geochemical soil profile) are analyzed to illustrate the benefits of using bilinear projection scores for variographic characterization instead of using individual variables. By using absolute variograms on a validated number of component scores, it is possible to make a combined multivariate chemometrics–variogram characterization of heterogeneous processes and...
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Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction
Published: Aug 2, 2013
Author(s): Woong Cho, Joon‐Ho Maeng, Yoomin Ahn, Seung Yong Hwang
Journal: ELECTROPHORESIS
This paper reports the development of a disposable, integrated biochip for DNA sample preparation and PCR. The hybrid biochip (25 × 45 mm) is composed of a disposable PDMS layer with a microchannel chamber and reusable glass substrate integrated with a microheater and thermal microsensor. Lysis, purification, and PCR can be performed sequentially on this microfluidic device. Cell lysis is achieved by heat and purification is...
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Enantiomeric separation of amlodipine and its two chiral impurities by nano‐liquid chromatography and capillary electrochromatography using a chiral stationary phase based on cellulose tris(4‐chloro‐3‐methylphenylcarbamate)
Published: Aug 2, 2013
Author(s): Roberta Auditore, Natale A. Santagati, Zeineb Aturki, Salvatore Fanali
Journal: ELECTROPHORESIS
In this work, a novel polysaccharide‐based chiral stationary phase, cellulose tris(4‐chloro‐3‐methylphenylcarbamate), also called Sepapak 4 has been evaluated for the chiral separation of amlodipine (AML) and its two impurities. AML is a powerful vasodilatator drug used for the treatment of hypertension. Capillary columns of 100 μm id packed with the chiral stationary phase were used for both nano‐LC and CEC experiments. The optimization...
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Aberrant proteins in the saliva of patients with oral squamous cell carcinoma
Published: Aug 2, 2013
Author(s): Kala Jessie, Jaime Jacqueline Jayapalan, Kien‐Chai Ong, Zubaidah Haji Abdul Rahim, Rosnah Mohd Zain, Kum‐Thong Wong, Onn Haji Hashim
Journal: ELECTROPHORESIS
Confirmation of oral squamous cell cancer (OSCC) currently relies on histological analysis, which does not provide clear indication of cancer development from precancerous lesions. In the present study, whole saliva proteins of patients with OSCC (n = 12) and healthy subjects (n = 12) were separated by 2DE to identify potential candidate biomarkers that are much needed to improve detection of the cancer. The OSCC patients’ 2DE saliva protein profiles...
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Method comparison on estimation for an unknown specimen in a heteroscedastic controlled calibration model
Published: Aug 1, 2013
Author(s): Jia‐Ren Tsai, Chen‐Tuo Liao
Journal: Journal of Chemometrics
In analytical chemistry applications, statistical calibration models are commonly used to estimate the true value of an unknown specimen. In this article, we consider a heteroscedastic controlled calibration model in which both dependent and independent variables are subject to heteroscedastic measurement errors. The main task of using this model is to estimate the true value of an unknown regressor (independent variable) under the...
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Adsorption of buffer ion pairs can alter long‐term electroosmotic flow stability
Published: Aug 1, 2013
Author(s): Ashley L. Morris, Christopher R. Harrison
Journal: ELECTROPHORESIS
Dynamic capillary coatings have become widespread due to their efficacy in modifying the EOF in capillary electrophoretic separations and ability to limit unwanted analyte‐surface interactions. However, our understanding of exactly what types of interactions are taking place at the surface of a capillary when these dynamic additives are present is limited. In this work, we have chosen a simple, small molecule additive, tetramethylammonium...
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MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross‐linked polyacrylamide gels
Published: Aug 1, 2013
Author(s): Dimitrios G. Papasotiriou, Stavroula Markoutsa, Jan Gorka, Enrico Schleiff, Michael Karas, Bjoern Meyer
Journal: ELECTROPHORESIS
Although the extraction of intact proteins from polyacrylamide gels followed by mass spectrometric molecular mass determination has been shown to be efficient, there is room for alternative approaches. Our study evaluates ethylene glycol diacrylate, a cleavable cross‐linking agent used for a new type of dissolvable gels. It attains an ester linkage that can be hydrolyzed in alkali conditions. The separation performance of the new gel...
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Graphene nanoparticles as pseudostationary phase for the electrokinetic separation of nonsteroidal anti‐inflammatory drugs
Published: Aug 1, 2013
Author(s): Sandra Benítez‐Martínez, Bartolomé M. Simonet, Miguel Valcárcel
Journal: ELECTROPHORESIS
The exceptional properties of graphene (G) were exploited here to facilitate capillary electrokinetic separations. Two types of commercially available G consisting of nanoparticles containing—one to three and—four to six G sheets, respectively, were compared for this purpose. Both proved effective in separating the arylpropyl derivatives of nonsteroidal anti‐inflammatory drugs. The highest resolution and shortest migration times were...
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