EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:


Please check back soon.

Wiley Online Library

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

Proteomics analysis of human pericardial fluid

Published: Aug 5, 2013

Author(s): Fei Xiang, Xuejiang Guo, Wen Chen, Jing Wang, Tao Zhou, Fuhua Huang, Changchun Cao, Xin Chen

Journal: PROTEOMICS

Pericardial fluid (PF) is considered as a biochemical window of heart. To date, there have been limited attempts to perform an in‐depth analysis of the PF proteome. In this study, an SDS‐PAGE‐LC‐MS/MS platform was utilized to explore depleted PF, which showed great coverage of low‐abundant proteins. In total, 1007 nonredundant proteins were identified with at least two peptides. This is the first comprehensive analysis of human PF...

Read More thumbnail image: Proteomics analysis of human pericardial fluid

Shotgun proteomic analysis of the Bombyx mori anterior silk gland: An insight into the biosynthetic fiber spinning process

Published: Aug 5, 2013

Author(s): Qiying Yi, Ping Zhao, Xin Wang, Yong Zou, Xiaowu Zhong, Chen Wang, Zhonghuai Xiang, Qing‐you Xia

Journal: PROTEOMICS

The Bombyx mori anterior silk gland (ASG) is a natural fiber manipulator for the material provided by the middle and posterior silk glands. In view of the significant role of the ASG in the liquid–crystal spinning process, a shotgun proteomics approach was taken to study the relationship between the function of proteins in the silkworm ASG and the spinning mechanism. A total of 1132 proteins with 7647 unique peptides were identified in...

Read More thumbnail image: Shotgun proteomic analysis of the Bombyx mori anterior silk gland An insight into the biosynthetic fiber spinning process

Design and evaluation of capillary coupled with optical fiber light‐emitting diode induced fluorescence detection for capillary electrophoresis

Published: Aug 5, 2013

Author(s): Hongyun Ji, Meng Li, Lihong Guo, Hongyan Yuan, Chunling Wang, Dan Xiao

Journal: ELECTROPHORESIS

A new detector, capillary coupled with optical fiber LED‐induced fluorescence detector (CCOF‐LED‐IFD, using CCOF for short), is introduced for CE. The strategy of the present work was that the optical fiber and separation capillary were, in the parallel direction, fastened in a fixation capillary with larger inner diameter. By employing larger inner diameter, the fixation capillary allowed the large diameter of the optical fiber to be...

Read More thumbnail image: Design and evaluation of capillary coupled with optical fiber lightemitting diode induced fluorescence detection for capillary electrophoresis

LC‐MS/MS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study

Published: Aug 5, 2013

Author(s): Xiaolin Wang, Dan Zhang, Man Liu, Hongna Zhao, Aihua Du, Lingjie Meng, Huichen Liu

Journal: Biomedical Chromatography

ABSTRACT A sensitive and selective LC‐MS/MS method for the determination of agomelatine in human plasma was developed and validated. After simple liquid–liquid extraction, the analytes were separated on a Zorbax SB‐C18 column (150 × 2.1 mm i.d., 5 µm) with an isocratic mobile phase consisting of 5 mm ammonium acetate solution (containing 0.1% formic acid) and methanol (30:70, v/v) at a flow‐rate of 0.3 mL/min. The MS acquisition was performed...

Read More thumbnail image: LCMSMS method for the determination of agomelatine in human plasma and its application to a pharmacokinetic study

Low‐energy ion scattering: Determining overlayer thickness for functionalized gold nanoparticles

Published: Aug 5, 2013

Author(s): Ali Rafati, Rik Veen, David G. Castner

Journal: Surface and Interface Analysis

With the widespread use of engineered nanoparticles for biomedical applications, detailed surface characterization is essential for ensuring reproducibility and the quality/suitability of the surface chemistry to the task at hand. One important surface property to be quantified is the overlayer thickness of self‐assembled monolayer (SAM) functionalized nanoparticles, as this information provides insight into SAM ordering and assembly. We...

Read More thumbnail image: Lowenergy ion scattering Determining overlayer thickness for functionalized gold nanoparticles

Development and optimization of on‐line 2‐dimensional chromatographic approaches for eliminating matrix effects and improving bioanalysis of peptides in human plasma using UHPLC‐MS/MS

Published: Aug 5, 2013

Author(s): Omnia A. Ismaiel, Rand G. Jenkins

Journal: Drug Testing and Analysis

Online 2‐dimensional chromatographic approaches for eliminating matrix effects and optimizing bioanalysis of peptides using ultra‐high performance liquid chromatography‐tandem mass spectrometry (UHPLC‐MS/MS) were studied. Three therapeutic peptides (octreotide, desmopressin, and vasopressin) were selected as model analytes. Human plasma was precipitated with acetonitrile; peptides were analyzed on C8, C18, Phenyl and HILIC ACQUITY UPLC...

Read More thumbnail image: Development and optimization of online 2dimensional chromatographic approaches for eliminating matrix effects and improving bioanalysis of peptides in human plasma using UHPLCMSMS

Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata (Benth.) Kudo by a macroporous resin column combined with high‐speed counter‐current chromatography

Published: Aug 2, 2013

Author(s): Hui‐Lan Yue, Xiao‐Hui Zhao, Li‐Juan Mei, Yun Shao

Journal: Journal of Separation Science

Five phenylethanoid glycosides (PhGs), forsythoside B, verbascoside, alyssonoside, isoverbascoside, and leucosceptoside B, were isolated and purified from Lamiophlomis rotata (Benth.) Kudo by high‐speed counter‐current chromatography (HSCCC) combined with macroporous resin (MR) column separation. In the present study, the two‐phase solvent system composed of ethyl acetate/n‐butanol/water (13:3:10, v/v/v) was used for HSCCC separation. A total of 27...

Read More thumbnail image: Separation and purification of five phenylpropanoid glycosides from Lamiophlomis rotata Benth_ Kudo by a macroporous resin column combined with highspeed countercurrent chromatography

Organic monoliths for high‐performance reversed‐phase liquid chromatography

Published: Aug 2, 2013

Author(s): Kun Liu, Pankaj Aggarwal, John S. Lawson, H. Dennis Tolley, Milton L. Lee

Journal: Journal of Separation Science

RPLC is the most common mode of LC. It is widely used for separations of both small and large molecules. Monolithic columns, which are currently under intensive study by many groups, have the potential of becoming attractive alternatives to particle‐packed columns. They are generally easier and faster to fabricate, and they demonstrate a lower pressure drop, less nonspecific adsorption, and richer chemistry (in the case of organic polymer...

Read More thumbnail image: Organic monoliths for highperformance reversedphase liquid chromatography

Ultra‐high performance size‐exclusion chromatography of synthetic polymers

Published: Aug 2, 2013

Author(s): Miroslav Janco, James N. Alexander, Edouard S. P. Bouvier, Damian Morrison

Journal: Journal of Separation Science

Ultra‐high performance size‐exclusion chromatography (UHP SEC) is a newly developed disruptive technology that allows the high‐resolution separation of synthetic polymers in as little as 2 min. The capability of UHP SEC for the characterization of synthetic polymers in organic solvents has been demonstrated. Using the Waters ACQUITY UPLC®1 H‐Class system and ethylene‐bridged hybrid size‐exclusion chromatography (SEC) columns packed with 1.7 to 2.5‐μm...

Read More thumbnail image: Ultrahigh performance sizeexclusion chromatography of synthetic polymers

Evaluation of the combinative application of SDS and sodium deoxycholate to the LCMS ‐based shotgun analysis of membrane proteomes

Published: Aug 2, 2013

Author(s): Yong Lin, Kunbo Wang, Yujun Yan, Haiyan Lin, Bin Peng, Zhonghua Liu

Journal: Journal of Separation Science

SDS and sodium deoxycholate (SDC) as two representative detergents have been widely used in LC–MS/MS‐based shotgun analysis of membrane proteomes. However, some inherent disadvantages limit their applications such as interference with MS analysis or their weak ability to disrupt membranes. To address this, the combinative application of SDS and SDC was developed and evaluated in our study, which comprehensively used the strong ability of...

Read More thumbnail image: Evaluation of the combinative application of SDS and sodium deoxycholate to the LC  MS based shotgun analysis of membrane proteomes

Retention behavior of isomeric triacylglycerols in silver‐ion HPLC : E ffects of mobile phase composition and temperature

Published: Aug 2, 2013

Author(s): Miroslav Lísa, Rumen Denev, Michal Holčapek

Journal: Journal of Separation Science

A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver‐ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using the most widespread hexane‐ and dichloromethane‐based mobile phases. The randomization of mono‐acyl TG standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of TG isomers. The mobile phase composition has no influence on...

Read More thumbnail image: Retention behavior of isomeric triacylglycerols in silverion HPLC  E ffects of mobile phase composition and temperature

Visualization and establishment of partial uniqueness and uniqueness rules in parallel factor analysis

Published: Aug 2, 2013

Author(s): Nematollah Omidikia, Hamid Abdollahi, Mohsen Kompany‐Zareh

Journal: Journal of Chemometrics

Parallel factor analysis (PARAFAC) is believed to be one of the most widely used multiway curve resolution techniques. The superiority of PARAFAC is due to its uniqueness property that is provided under mild assumptions. Unambiguously recovered chemically and physically meaningful profiles by PARAFAC are reliable for quantitative and qualitative application. The essential uniqueness condition of PARAFAC breaks totally or partially in data...

Read More thumbnail image: Visualization and establishment of partial uniqueness and uniqueness rules in parallel factor analysis

Multivariate variographic versus bilinear data modeling

Published: Aug 2, 2013

Author(s): Pentti Minkkinen, Kim Harry Esbensen

Journal: Journal of Chemometrics

Two contrasting multivariate data sets (a process data series vs. a 1‐D geochemical soil profile) are analyzed to illustrate the benefits of using bilinear projection scores for variographic characterization instead of using individual variables. By using absolute variograms on a validated number of component scores, it is possible to make a combined multivariate chemometrics–variogram characterization of heterogeneous processes and...

Read More thumbnail image: Multivariate variographic versus bilinear data modeling

Disposable on‐chip microfluidic system for buccal cell lysis, DNA purification, and polymerase chain reaction

Published: Aug 2, 2013

Author(s): Woong Cho, Joon‐Ho Maeng, Yoomin Ahn, Seung Yong Hwang

Journal: ELECTROPHORESIS

This paper reports the development of a disposable, integrated biochip for DNA sample preparation and PCR. The hybrid biochip (25 × 45 mm) is composed of a disposable PDMS layer with a microchannel chamber and reusable glass substrate integrated with a microheater and thermal microsensor. Lysis, purification, and PCR can be performed sequentially on this microfluidic device. Cell lysis is achieved by heat and purification is...

Read More thumbnail image: Disposable onchip microfluidic system for buccal cell lysis DNA purification and polymerase chain reaction

Enantiomeric separation of amlodipine and its two chiral impurities by nano‐liquid chromatography and capillary electrochromatography using a chiral stationary phase based on cellulose tris(4‐chloro‐3‐methylphenylcarbamate)

Published: Aug 2, 2013

Author(s): Roberta Auditore, Natale A. Santagati, Zeineb Aturki, Salvatore Fanali

Journal: ELECTROPHORESIS

In this work, a novel polysaccharide‐based chiral stationary phase, cellulose tris(4‐chloro‐3‐methylphenylcarbamate), also called Sepapak 4 has been evaluated for the chiral separation of amlodipine (AML) and its two impurities. AML is a powerful vasodilatator drug used for the treatment of hypertension. Capillary columns of 100 μm id packed with the chiral stationary phase were used for both nano‐LC and CEC experiments. The optimization...

Read More thumbnail image: Enantiomeric separation of amlodipine and its two chiral impurities by nanoliquid chromatography and capillary electrochromatography using a chiral stationary phase based on cellulose tris4chloro3methylphenylcarbamate

Aberrant proteins in the saliva of patients with oral squamous cell carcinoma

Published: Aug 2, 2013

Author(s): Kala Jessie, Jaime Jacqueline Jayapalan, Kien‐Chai Ong, Zubaidah Haji Abdul Rahim, Rosnah Mohd Zain, Kum‐Thong Wong, Onn Haji Hashim

Journal: ELECTROPHORESIS

Confirmation of oral squamous cell cancer (OSCC) currently relies on histological analysis, which does not provide clear indication of cancer development from precancerous lesions. In the present study, whole saliva proteins of patients with OSCC (n = 12) and healthy subjects (n = 12) were separated by 2DE to identify potential candidate biomarkers that are much needed to improve detection of the cancer. The OSCC patients’ 2DE saliva protein profiles...

Read More thumbnail image: Aberrant proteins in the saliva of patients with oral squamous cell carcinoma

Method comparison on estimation for an unknown specimen in a heteroscedastic controlled calibration model

Published: Aug 1, 2013

Author(s): Jia‐Ren Tsai, Chen‐Tuo Liao

Journal: Journal of Chemometrics

In analytical chemistry applications, statistical calibration models are commonly used to estimate the true value of an unknown specimen. In this article, we consider a heteroscedastic controlled calibration model in which both dependent and independent variables are subject to heteroscedastic measurement errors. The main task of using this model is to estimate the true value of an unknown regressor (independent variable) under the...

Read More thumbnail image: Method comparison on estimation for an unknown specimen in a heteroscedastic controlled calibration model

Adsorption of buffer ion pairs can alter long‐term electroosmotic flow stability

Published: Aug 1, 2013

Author(s): Ashley L. Morris, Christopher R. Harrison

Journal: ELECTROPHORESIS

Dynamic capillary coatings have become widespread due to their efficacy in modifying the EOF in capillary electrophoretic separations and ability to limit unwanted analyte‐surface interactions. However, our understanding of exactly what types of interactions are taking place at the surface of a capillary when these dynamic additives are present is limited. In this work, we have chosen a simple, small molecule additive, tetramethylammonium...

Read More thumbnail image: Adsorption of buffer ion pairs can alter longterm electroosmotic flow stability

MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate cross‐linked polyacrylamide gels

Published: Aug 1, 2013

Author(s): Dimitrios G. Papasotiriou, Stavroula Markoutsa, Jan Gorka, Enrico Schleiff, Michael Karas, Bjoern Meyer

Journal: ELECTROPHORESIS

Although the extraction of intact proteins from polyacrylamide gels followed by mass spectrometric molecular mass determination has been shown to be efficient, there is room for alternative approaches. Our study evaluates ethylene glycol diacrylate, a cleavable cross‐linking agent used for a new type of dissolvable gels. It attains an ester linkage that can be hydrolyzed in alkali conditions. The separation performance of the new gel...

Read More thumbnail image: MALDI analysis of proteins after extraction from dissolvable ethylene glycol diacrylate crosslinked polyacrylamide gels

Graphene nanoparticles as pseudostationary phase for the electrokinetic separation of nonsteroidal anti‐inflammatory drugs

Published: Aug 1, 2013

Author(s): Sandra Benítez‐Martínez, Bartolomé M. Simonet, Miguel Valcárcel

Journal: ELECTROPHORESIS

The exceptional properties of graphene (G) were exploited here to facilitate capillary electrokinetic separations. Two types of commercially available G consisting of nanoparticles containing—one to three and—four to six G sheets, respectively, were compared for this purpose. Both proved effective in separating the arylpropyl derivatives of nonsteroidal anti‐inflammatory drugs. The highest resolution and shortest migration times were...

Read More thumbnail image: Graphene nanoparticles as pseudostationary phase for the electrokinetic separation of nonsteroidal antiinflammatory drugs
Page:   Prev 1 2 3 4 5 6 7 8 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2013 John Wiley & Sons, Inc. All Rights Reserved