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Comparison of four extraction methods for analysis of volatile hop‐derived aroma compounds in beer

Published: Oct 6, 2017

Author(s): Tobias M. Richter, Graham T. Eyres, Patrick Silcock, Phil J. Bremer

Journal: Journal of Separation Science

Abstract The volatile organic compound profile in beer is derived from hops, malt, yeast, and interactions between the ingredients, making it very diverse and complex. Due to the range and diversity of the volatile organic compounds present, the choice of the extraction method is extremely important for optimal sensitivity and selectivity. This study compared four extraction methods for hop‐derived compounds in beer late hopped with...

Read More thumbnail image: Comparison of four extraction methods for analysis of volatile hopderived aroma compounds in beer

High‐throughput LC–MS method for the rapid characterization of multiple chemical constituents and metabolites of Da‐Bu‐Yin‐Wan

Published: Oct 6, 2017

Author(s): Xianna Li, Hui Sun, Aihua Zhang, Zhidong Liu, Di Zou, Yanhua Song, Liang Liu, Xijun Wang

Journal: Journal of Separation Science

Abstract Traditional Chinese medicine is the clinical experience accumulated by Chinese people against diseases. Da‐Bu‐Yin‐Wan is a famous traditional Chinese medicine formula consisting of Phellodendri amurensis Rupr., Anemarrhenae asphodeloides Bge., Radix Rehmanniae Preparata and Chinemys reevesii. In this study, ultra high performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight high‐definition mass...

Read More thumbnail image: Highthroughput LCMS method for the rapid characterization of multiple chemical constituents and metabolites of DaBuYinWan

Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities

Published: Oct 4, 2017

Author(s): N. M. El Zahar, N. Magdy, A. M. El‐Kosasy, Michael G. Bartlett

Journal: Biomedical Chromatography

Abstract Phosphorothioate (PS) oligonucleotides are a rapidly rising class of drugs with significant therapeutic applications. However, owing to their complex structure and multistep synthesis and purification processes, generation of low‐level impurities and degradation products are common. Therefore, they require significant investment in quality control and impurity identification. This requires the development of advanced...

Read More thumbnail image: Chromatographic approaches for the characterization and quality control of therapeutic oligonucleotide impurities

Hydrophilic interaction liquid chromatography tandem mass spectrometry analysis of malonyl‐coenzyme A in breast cancer cell cultures applying online solid‐phase extraction

Published: Oct 4, 2017

Author(s): Alexander Schriewer, Cristina Cadenas, Heiko Hayen

Journal: Journal of Separation Science

Abstract Cofactors such as coenzyme A and its derivatives acetyl‐coenzyme A and malonyl‐coenzyme A are involved in many metabolic pathways. Due to trace level concentrations in biological samples and the high reactivity of cofactors, a fast, sensitive, and selective method for quantification is mandatory. In this study, online solid‐phase extraction was coupled successfully to hydrophilic interaction liquid chromatography with...

Read More thumbnail image: Hydrophilic interaction liquid chromatography tandem mass spectrometry analysis of malonylcoenzyme A in breast cancer cell cultures applying online solidphase extraction

Synthesis of monodisperse silica microspheres and modification with diazoresin for mixed‐mode ultra high performance liquid chromatography separations

Published: Oct 4, 2017

Author(s): Hailin Cong, Bing Yu, Chao Tian, Shuai Zhang, Hua Yuan

Journal: Journal of Separation Science

Abstract Monodisperse silica particles with average diameters of 1.9–2.9 μm were synthesized by a modified Stöber method, in which tetraethyl orthosilicate was continuously supplied to the reaction mixture containing KCl electrolyte, water, ethanol, and ammonia. The obtained silica particles were modified by self‐assembly with positively charged photosensitive diazoresin on the surface. After treatment with ultraviolet light, the...

Read More thumbnail image: Synthesis of monodisperse silica microspheres and modification with diazoresin for mixedmode ultra high performance liquid chromatography separations

Solvent‐vapor‐assisted liquid–liquid microextraction: A novel method for the determination of phthalate esters in aqueous samples using GC–MS

Published: Oct 4, 2017

Author(s): Maryam Mohebbi, Rouhollah Heydari, Majid Ramezani

Journal: Journal of Separation Science

Abstract A novel liquid–liquid microextraction method, namely, solvent‐vapor‐assisted liquid–liquid microextraction for the determination of dimethyl phthalate, diethyl phthalate, dibutyl phthalate and bis(2‐ethylhexyl) phthalate in the aqueous samples using gas chromatography with mass spectrometry was developed. In the proposed method, extracting solvent was heated, and solvent vapor as the extracting phase was injected into the...

Read More thumbnail image: Solventvaporassisted liquidliquid microextraction A novel method for the determination of phthalate esters in aqueous samples using GCMS

High‐performance liquid chromatography thermodynamic study of new potential antiepileptic compounds on a cholesterol column using isocratic elution with methanol/water and acetonitrile/water eluent systems

Published: Oct 4, 2017

Author(s): Jolanta Flieger, Hanna Trębacz, Magdalena Pizoń, Anna Kowalska, Agnieszka Szczęsna, Tomasz Plech

Journal: Journal of Separation Science

Abstract Basic thermodynamic functions responsible for retention of new 1,2,4‐triazole derivatives exhibiting varied antiepileptic activity on cholesterol‐based stationary phase were determined. Evaluation of the Gibbs energy change, the change in enthalpy and the change in entropy was based on the van't Hoff relationship representing lnk versus 1/T. A detailed discussion of the van't Hoff equation, exploring the influence of the phase ratio on...

Read More thumbnail image: Highperformance liquid chromatography thermodynamic study of new potential antiepileptic compounds on a cholesterol column using isocratic elution with methanolwater and acetonitrilewater eluent systems

Affinity capillary electrophoresis for the assessment of binding affinity of carbohydrate‐based cholera toxin inhibitors

Published: Oct 4, 2017

Author(s): Oier Aizpurua‐Olaizola, Javier Sastre Torano, Aliaksei Pukin, Ou Fu, Geert Jan Boons, Gerhardus J. Jong, Roland J. Pieters

Journal: ELECTROPHORESIS

Abstract Developing tools for the study of protein carbohydrate interactions is an important goal in glycobiology. Cholera toxin inhibition is an interesting target in this context, as its inhibition may help to fight against cholera. For the study of novel ligands an affinity capillary electrophoresis (ACE) method was optimized and applied. The method uses unlabeled cholera toxin B‐subunit (CTB) and unlabeled carbohydrate ligands...

Read More thumbnail image: Affinity capillary electrophoresis for the assessment of binding affinity of carbohydratebased cholera toxin inhibitors

Screening bioactive compounds from natural product and its preparations using capillary electrophoresis

Published: Oct 4, 2017

Author(s): Huifen Ma, Yun Bai, Jin Li, Yan‐xu Chang

Journal: ELECTROPHORESIS

Abstract Bioactive ingredients of natural products can protect human body from harm, as well as prevent and treat disease. Screening bioactive compounds from natural products is attracting particular attention. It is a great challenge to separate and detect the active compounds from complex matrix natural products. CE plays a vital role in screening active compounds because of its unique features such as high‐efficiency separation,...

Read More thumbnail image: Screening bioactive compounds from natural product and its preparations using capillary electrophoresis

Cultivation and quantitative single‐cell analysis of Saccharomyces cerevisiae on a multifunctional microfluidic device

Published: Oct 4, 2017

Author(s): Simone Stratz, Pascal Emilio Verboket, Karina Hasler, Petra Stephanie Dittrich

Journal: ELECTROPHORESIS

Abstract Here, we present a multifunctional microfluidic device whose integrative design enables to combine cell culture studies and quantitative single cell biomolecule analysis. The platform consists of 32 analysis units providing two key features; first, a micrometer‐sized trap for hydrodynamic capture of a single Saccharomyces cerevisiae (S. cerevisiae) yeast cell; second, a convenient double‐valve configuration surrounding the trap....

Read More thumbnail image: Cultivation and quantitative singlecell analysis of Saccharomyces cerevisiae on a multifunctional microfluidic device

Effect of pore size on performance of monolithic tube chromatography of large biomolecules

Published: Oct 4, 2017

Author(s): Ales Podgornik, Masataka Hamachi, Yu Isakari, Noriko Yoshimoto, Shuichi Yamamoto

Journal: ELECTROPHORESIS

Abstract Effect of pore size on the performance of ion‐exchange monolith tube chromatography of large biomolecules was investigated. Radial flow 1 mL polymer based monolith tubes of different pore sizes (1.5, 2, and 6 μm) were tested with model samples such as 20 mer poly T‐DNA, basic proteins, and acidic proteins (molecular weight 14 000–670 000). Pressure drop, pH transient, the number of binding site, dynamic...

Read More thumbnail image: Effect of pore size on performance of monolithic tube chromatography of large biomolecules

Electrokinetic motion of a spherical micro particle at an oil−water interface in microchannel

Published: Oct 4, 2017

Author(s): Chengfa Wang, Mengqi Li, Yongxin Song, Xinxiang Pan, Dongqing Li

Journal: ELECTROPHORESIS

Abstract The electrokinetic motion of a negatively charged spherical particle at an oil−water interface in a microchannel is numerically investigated and analyzed in this paper. A three−dimensional (3D) transient numerical model is developed to simulate the particle electrokinetic motion. The channel wall, the surface of the particle and the oil−water interface are all considered negatively charged. The effects of the direct current...

Read More thumbnail image: Electrokinetic motion of a spherical micro particle at an oilwater interface in microchannel

Organic polymer‐based monolithic stationary phases with incorporated nanostructured materials for HPLC and CEC

Published: Oct 4, 2017

Author(s): Nisansala Ganewatta, Ziad El Rassi

Journal: ELECTROPHORESIS

Abstract This review article is concerned with the recent advances made in the field of organic polymer‐based monoliths with incorporated nanostructured materials (NSMs) for use in liquid chromatography and capillary electrochromatography. It covers the pertinent literature published over the last 7–8 years with a total of 56 references. The present article has two distinct parts: one major part encompassing “traditional” organic...

Read More thumbnail image: Organic polymerbased monolithic stationary phases with incorporated nanostructured materials for HPLC and CEC

Anti‐inflammatory activities and glycerophospholipids metabolism in KLA‐stimulated RAW 264.7 macrophage cells by diarylheptanoids from the rhizomes of Alpinia officinarum

Published: Oct 2, 2017

Author(s): Guogai Zhang, Lifang Zhao, Jiancheng Zhu, Yifan Feng, Xia Wu

Journal: Biomedical Chromatography

Abstract Alpinia officinarum is used for its anti‐inflammatory activity historically in China. Diarylheptanoids isolated from A. officinarum play important biological roles in the prevention and treatment of inflammatory disorders. Seven diarylheptanoids (1–7) were isolated from A. officinarum. The cell viabilities and anti‐inflammatory activities of diarylheptanoids were evaluated by MTT assay and tumor necrosis factor‐α production in...

Read More thumbnail image: Antiinflammatory activities and glycerophospholipids metabolism in KLAstimulated RAW 264_7 macrophage cells by diarylheptanoids from the rhizomes of Alpinia officinarum

Simultaneous determination of baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re of Banxia xiexin decoction in rat plasma by LC–MS/MS and its application to a pharmacokinetic study

Published: Oct 2, 2017

Author(s): Ying Wang, Yifan Zhang, Juan Xiao, Ranchi Xu, Qiangli Wang, Xinhong Wang

Journal: Biomedical Chromatography

Abstract A rapid and high‐sensitivity liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed for simultaneous determination of nine active constituents (baicalin, baicalein, wogonoside, wogonin, scutellarin, berberine, coptisine, ginsenoside Rb1 and ginsenoside Re) in rat plasma after oral administration of Banxia xiexin decoction (BXD). Biological samples were processed wtih acetone–ethyl acetate (4:1, v/v)....

Read More thumbnail image: Simultaneous determination of baicalin baicalein wogonoside wogonin scutellarin berberine coptisine ginsenoside Rb1 and ginsenoside Re of Banxia xiexin decoction in rat plasma by LCMSMS and its application to a pharmacokinetic study

Simple determination of betaine, l ‐carnitine and choline in human urine using self‐packed column and column‐switching ion chromatography with nonsuppressed conductivity detection

Published: Oct 2, 2017

Author(s): Dan Wei, Yan Zhu, Ming Guo

Journal: Biomedical Chromatography

Abstract A sequential online extraction, clean‐up and separation system for the determination of betaine, l‐carnitine and choline in human urine using column‐switching ion chromatography with nonsuppressed conductivity detection was developed in this work. A self‐packed pretreatment column (50 × 4.6 mm, i.d.) was used for the extraction and clean‐up of betaine, l‐carnitine and choline. The separation was achieved using self‐packed cationic...

Read More thumbnail image: Simple determination of betaine l carnitine and choline in human urine using selfpacked column and columnswitching ion chromatography with nonsuppressed conductivity detection

Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLC–MS/MS

Published: Oct 2, 2017

Author(s): Pravin G. Vanol, Manish Yadav, Mallika Sanyal, Priyanka A. Shah, Pranav S. Shrivastav

Journal: Biomedical Chromatography

Abstract An improved, precise and reliable ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method has been developed for the quantification of trimetazidine, using trimetazidine‐d8 as the internal standard (IS). Interference owing to plasma phospholipids during sample preparation was overcome using a hybrid solid‐phase extraction–phospholipid ultra cartridge. The mean extraction recovery of...

Read More thumbnail image: Overcoming interference of plasma phospholipids using HybridSPE for the determination of trimetazidine by UPLCMSMS

Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP‐UPLC–MS complementary with HILIC‐UPLC–MS technique

Published: Oct 2, 2017

Author(s): Lang Lang, Zhaorui Meng, Lan Sun, Wei Xiao, Longshan Zhao, Zhili Xiong

Journal: Biomedical Chromatography

Abstract Guizhi Fuling capsule (GFC), developed from the traditional Chinese prescription of Guizhi Fuling Wan, has been commonly used for the treatment of primary dysmenorrhea (PD). However, the intervention effective mechanism in vivo has not been well elucidated. In this study, an integrated plasma metabonomic strategy based on RP‐UPLC‐MS coupled with HILIC‐UPLC‐MS technique has been developed to investigate the global therapeutic effects and...

Read More thumbnail image: Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RPUPLCMS complementary with HILICUPLCMS technique

Real‐time metabolite monitoring of glucose‐fed Clostridium acetobutylicum fermentations using Raman assisted metabolomics

Published: Oct 2, 2017

Author(s): Theresah N.K. Zu, Sanchao Liu, Elliot S. Gerlach, Katherine L. Germane, Matthew D. Servinsky, David M. Mackie, Christian J. Sund

Journal: Journal of Raman Spectroscopy

Abstract Data obtained from in situ Raman spectroscopy probes and high‐performance liquid chromatography (HPLC) analysis were applied together with chemometrics to build partial least squares models of metabolite concentrations for the industrially relevant organism Clostridium acetobutylicum. Models were built for predominant products (acetic acid, butyric acid, and butanol) of C. acetobutylicum cultures grown on glucose as a substrate....

Read More thumbnail image: Realtime metabolite monitoring of glucosefed Clostridium acetobutylicum fermentations using Raman assisted metabolomics

Raman mapping of the S 3 chromophore in degraded ultramarine blue paints

Published: Oct 2, 2017

Author(s): Eleanor Cato, Nadim Scherrer, Ester S.B. Ferreira

Journal: Journal of Raman Spectroscopy

Abstract A specific case of degradation was observed in synthetic ultramarine paint within 3 paintings from the early 20th century, manifesting as an intricate pattern of white lines criss‐crossing the blue paint surface. Raman spectroscopy can be performed directly on untreated sample surfaces and is sensitive to the colour components in ultramarine (S3− and S2− chromophores). This method was chosen to map the chromophore intensity...

Read More thumbnail image: Raman mapping of the S 3  chromophore in degraded ultramarine blue paints
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