EarlyView Articles

X chromosomal recombination—A family study analyzing 26 X ‐ STR L oci in C hinese H an three‐generation pedigrees

Published: Oct 3, 2013

Author(s): Qiu‐Ling Liu, Ze‐Dong Li, Cheng‐Tao Li, Hu Zhao, Ye‐Da Wu, Quan Li, De‐Jian Lu

Journal: ELECTROPHORESIS

The aim of this study is to investigate genetic linkage and recombination fractions of 26 X chromosomal (X‐STR) loci with two multiplex PCR systems (MX15‐STR and MX12‐STR). MX15‐STR (including DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074, and DXS10079) and MX12‐STR (including DXS6854, DXS9902, DXS6800, GATA172D05, DXS7423, HPRTB, DXS6807, DXS6803,...

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Published: Oct 2, 2013

Author(s):

Journal: Journal of Chemometrics

No abstract is available for this article.

Perspectives in process analysis

Published: Oct 2, 2013

Author(s): Rudolf W. Kessler

Journal: Journal of Chemometrics

Separation behavior of short single‐ and double‐stranded DNA in 1 micron and 100 nm glass channels

Published: Oct 2, 2013

Author(s): Alexander J. Russell, Jackson T. Bonis‐O'Donnell, Thomas M. Wynne, Maria T. Napoli, Sumita Pennathur

Journal: ELECTROPHORESIS

Micro‐ and nanofluidic lab‐on‐chip technology offers the unique capability of high‐resolution separation, identification, and manipulation of biomolecules with broad applications in chemistry, biology, and medicine. In this work, we probe the effects of ionic strength on separation of ss‐ and dsDNA within 1 micron and 100 nm‐deep glass channels. Separation behavior of DNA is influenced by a number of parameters, including ionic strength,...

Rapid determination of partition coefficients of pharmaceuticals by phase distribution and microchip capillary electrophoresis with contactless conductivity detection

Published: Oct 1, 2013

Author(s): Sifeng Wang, Zuanguang Chen, Xiuwen Tang, Lijuan Shi, Lin Zhang, Meicun Yao

Journal: Journal of Separation Science

A simple microchip CE method integrated with contactless conductivity detection was developed for the direct determination of partition coefficients of selected pharmaceuticals after phase distribution equilibrium. The equilibrium of distribution between two phases for four pharmaceuticals was performed using a 1‐octanol/water system and 1‐octanol/buffer system. During the concentration determination, several major factors affecting...

SPE of 5‐lipoxygenase metabolites and the effect of head‐column field‐amplified sample stacking in MEKC

Published: Oct 1, 2013

Author(s): Hans Abromeit, Fengyi Wu, Gerhard K. E. Scriba

Journal: Journal of Separation Science

The SPE of leukotrienes and eicosatetraenoic acids using anion exchange materials was compared to the classical extraction with C18 columns. A silica‐based strong anion exchanger, a polymer‐based weak anion exchanger, and a polymer‐based mixed‐mode strong anion exchanger were studied. All anion exchange materials displayed a higher recovery of the analytes with values between 70 and 90% when extracting standard solutions and analyzing by...

Column comparison and method development for the analysis of short‐chain carboxylic acids by zwitterionic hydrophilic interaction liquid chromatography with UV detection

Published: Oct 1, 2013

Author(s): Giorgio Marrubini, Alice Pedrali, Petrus Hemström, Tobias Jonsson, Patrik Appelblad, Gabriella Massolini

Journal: Journal of Separation Science

Short‐chain carboxylic acids are relevant in pharmaceutical, food quality control, and biomedical analysis. In this study, 11 acids commonly found in drugs and in food products were selected. Wine was chosen as matrix for testing the method. The test compounds were used for comparing the selectivity of four 150 × 2.1 mm zwitterionic hydrophilic interaction LC (HILIC) columns (ZIC‐HILIC 5 μm, 200 Å, and 3.5 μm, 100 Å, ZIC‐pHILIC 5 μm,...

Preparation and loading buffer study of polyvinyl alcohol‐based immobilized T i ⁴⁺ affinity chromatography for phosphopeptide enrichment

Published: Oct 1, 2013

Author(s): Yufeng Hu, Shuangxi Guo, Hongbo Ma, Ning Ye, Xueqin Ren

Journal: Journal of Separation Science

Despite recent advances in phosphoproteomics, an efficient and simple enrichment protocol is still a challenge and of high demand aiming at large‐scale plant phosphoproteomics studies. Here, we developed a novel loading buffer system for synthesized immobilized metal affinity chromatography material targeting plant samples, which was prepared by a simple one‐step esterification between polyvinyl alcohol and phosphoric acid and then was...

Ultrasound‐assisted magnetic SPE based on Fe ₃ O ₄ ‐grafted graphene for the determination of polychlorinated biphenyls in water samples

Published: Oct 1, 2013

Author(s): Xiaoji Cao, Jiaoyu Chen, Xuemin Ye, Feifei Zhang, Lingxiao Shen, Weimin Mo

Journal: Journal of Separation Science

An ultrasound‐assisted magnetic SPE procedure with an Fe3O4‐grafted graphene nanocomposite as the magnetic adsorbent has been developed to determine seven polychlorinated biphenyls (PCBs; PCB28, PCB52, PCB101, PCB118, PCB138, PCB153, and PCB180) simultaneously in 200 mL environmental water samples, in combination with GC–MS/MS. Several factors related to magnetic SPE efficiencies, such as the superparamagnetic intensity and amount of...

Combination of supercritical fluid extraction with counter‐current chromatography to isolate anthocyanidins from the petals of Chaenomeles sinensis based on mathematical calculations

Published: Oct 1, 2013

Author(s): Sainan Li, Liping Guo, Chunming Liu, Zi′ao Fu, Yuchi Zhang

Journal: Journal of Separation Science

Supercritical fluid extraction (SFE) coupled with high‐speed counter‐current chromatography (HSCCC) was successfully used for the extraction and on‐line isolation of the anthocyanidins from the petals of Chaenomeles sinensis in two stages. The SFE parameters were optimized by an orthogonal test, and the solvent systems of SFE and HSCCC were calculated and optimized with the help of a multiexponential function model. In the first stage, the lower...

Evaluation of ubiquitinated proteins by proteomics reveals the role of the ubiquitin proteasome system in the regulation of G rp75 and G rp78 chaperone proteins during intestinal inflammation

Published: Oct 1, 2013

Author(s): Julien Bertrand, Naouel Tennoune, Rachel Marion‐Letellier, Alexis Goichon, Philippe Chan, Khaly Mbodji, David Vaudry , Pierre Déchelotte, Moïse Coëffier

Journal: PROTEOMICS

The ubiquitin proteasome system (UPS) is the major pathway of intracellular protein degradation and may be involved in the pathophysiology of inflammatory bowel diseases or irritable bowel syndrome. UPS specifically degrades proteins tagged with an ubiquitin chain. We aimed to identify polyubiquitinated proteins during inflammatory response in intestinal epithelial HCT‐8 cells by a proteomic approach. HCT‐8 cells were incubated with...

Microscopic imaging mass spectrometry assisted by on‐tissue chemical derivatization for visualizing multiple amino acids in human colon cancer xenografts

Published: Oct 1, 2013

Author(s): Sakino Toue, Yuki Sugiura, Akiko Kubo, Mitsuyo Ohmura, Sachise Karakawa, Toshimi Mizukoshi, Junya Yoneda, Hiroshi Miyano, Yasushi Noguchi, Tsuyoshi Kobayashi, Yasuaki Kabe, Makoto Suematsu

Journal: PROTEOMICS

Imaging MS combined with CE/MS serves as a method to provide semi‐quantitative and spatial information of small molecular metabolites in tissue slices. However, not all metabolites including amino acids have fully been visualized, because of low‐ionization efficiency in MALDI MS. This study aimed to acquire semi‐quantitative spatial information for multiple amino acids in frozen tissue slices. As a derivatization reagent,...

Proteomic changes in chicken primary hepatocytes exposed to T ‐2 toxin are associated with oxidative stress and mitochondrial enhancement

Published: Oct 1, 2013

Author(s): Peiqiang Mu, Ming Xu, Lei Zhang, Kaixin Wu, Jun Wu, Jun Jiang, Qingmei Chen, Lijuan Wang, Xianqing Tang, Yiqun Deng

Journal: PROTEOMICS

T‐2 toxin is a mycotoxin that is toxic to plants, animals, and humans. However, its molecular mechanism remains unclear, especially in chickens. In this study, using 2D electrophoresis with MALDI‐TOF/TOF‐MS, 53 proteins were identified as up‐ or downregulated by T‐2 toxin in chicken primary hepatocytes. Functional network analysis by ingenuity pathway analysis showed that the top network altered by T‐2 toxin is associated with...

Proteome analysis of functionally differentiated bovine ( Bos indicus ) mammary epithelial cells isolated from milk

Published: Oct 1, 2013

Author(s): Jagadeesh Janjanam, Manu Jamwal, Surender Singh, Saravanan Kumar, Aswini K. Panigrahi, Gururao Hariprasad, Manoj K. Jena, Vijay Anand, Sudarshan Kumar, Jai K. Kaushik, Ajay K. Dang, Manishi Mukesh, Bishnu P. Mishra, Alagiri Srinivasan, Vanga S. Reddy, Ashok K. Mohanty

Journal: PROTEOMICS

Mammary gland is made up of a branching network of ducts that end in alveoli. Terminally differentiated mammary epithelial cells (MECs) constitute the innermost layer of aveoli. They are milk‐secreting cuboidal cells that secrete milk proteins during lactation. Little is known about the expression profile of proteins in the metabolically active MECs during lactation or their functional role in the lactation process. In the present...

2D DIGE based proteomics study of erythrocyte cytosol in sickle cell disease: Altered proteostasis and oxidative stress

Published: Oct 1, 2013

Author(s): Avik Basu, Sutapa Saha, Shilpita Karmakar, Sudipa Chakravarty, Debasis Banerjee, Bisnu Prasad Dash, Abhijit Chakrabarti

Journal: PROTEOMICS

Sickle cell disease (SCD) is a hemolytic disorder caused by a mutation in beta‐globin gene and affects millions of people worldwide. Though clinical manifestations of the disease are quite heterogeneous, many of them occur due to erythrocyte sickling at reduced oxygen concentration and vascular occlusion mediated via blood cell adhesion to the vessel wall. We have followed proteomic approach to resolve the differentially regulated...

Characterization of the EGFR interactome reveals associated protein complex networks and intracellular receptor dynamics

Published: Oct 1, 2013

Author(s): Sarah Foerster, Tim Kacprowski, Vishnu Mukund Dhople, Elke Hammer, Susann Herzog, Hisham Saafan, Sandra Bien‐Möller, Mario Albrecht, Uwe Völker, Christoph A. Ritter

Journal: PROTEOMICS

Growth factor receptor mediated signaling is meanwhile recognized as a complex signaling network, which is initiated by recruiting specific patterns of adaptor proteins to the intracellular domain of epidermal growth factor receptor (EGFR). Approaches to globally identify EGFR‐binding proteins are required to elucidate this network. We affinity‐purified EGFR with its interacting proteins by coprecipitation from lysates of A431 cells. A...

In‐plane alloy electrodes for capacitively coupled contactless conductivity detection in poly(methylmethacrylate) electrophoretic chips

Published: Oct 1, 2013

Author(s): Adam J. Gaudry, Michael C. Breadmore, Rosanne M. Guijt

Journal: ELECTROPHORESIS

A simple method for producing PMMA electrophoresis microchips with in‐plane electrodes for capacitively coupled contactless conductivity detection is presented. One PMMA plate (channel plate) is embossed with the microfluidic and electrode channels and lamination bonded to a blank PMMA cover plate of equal dimensions. To incorporate the electrodes, the bonded chip is heated to 80°C, above the melting point of the alloy (≈70°C) and below...

Development of the 16 X ‐ STR loci typing system and genetic analysis in a S hanghai H an population from C hina

Published: Oct 1, 2013

Author(s): Kuan Sun, Shumin Zhao, Huaizhou Tian, Suhua Zhang, Chengtao Li

Journal: ELECTROPHORESIS

This study developed a new multiplex PCR system that simultaneously amplifies 16 X‐STR loci in the same PCR reaction, and the polymorphism and mutation rates of these 16 X‐STR loci were explored in a Shanghai Han population from China. These loci included DXS10134, DXS10159, DXS6789, DXS6795, DXS6800, DXS6803, DXS6807, DXS6810, DXS7132, DXS7424, DXS8378, DXS9902, GATA165B12, GATA172D05, GATA31E08, and HPRTB. Samples from 591 unrelated...

Characterization of human papillomavirus by capillary isoelectric focusing with whole‐column imaging detection

Published: Oct 1, 2013

Author(s): Chao‐Ming Zhou

Journal: ELECTROPHORESIS

CIEF with whole‐column imaging detection (WCID) can be a useful tool for the characterization and identification of human papillomavirus (HPV). This article is the initial report of the determination of the pI of HPV by CIEF‐WCID method. In this study, components of the assay selected for optimization were ampholytes, additives, methylcellulose concentration, HPV concentration, salt concentration, and focusing time. Then the optimization CIEF‐WCID...

Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture

Published: Oct 1, 2013

Author(s): Zuzana Svobodova, Jana Kucerova, Julien Autebert, Daniel Horak, Lenka Bruckova, Jean‐Louis Viovy, Zuzana Bilkova

Journal: ELECTROPHORESIS

In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells—specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model...