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A comparative analysis of human plasma and serum proteins by combining native PAGE, whole‐gel slicing and quantitative LC‐MS/MS: Utilizing native MS‐electropherograms in proteomic analysis for discovering structure and interaction‐correlated differences

Published: Sep 26, 2017

Author(s): Meiling Wen, Ya Jin, Takashi Manabe, Shumin Chen, Wen Tan

Journal: ELECTROPHORESIS

Abstract MS identification has long been used for PAGE‐separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole‐gel slicing and quantitative LC‐MS/MS, aiming at comparative analysis on not only abundance, but also structures and interactions of proteins. A pair of human plasma and...

Read More thumbnail image: A comparative analysis of human plasma and serum proteins by combining native PAGE wholegel slicing and quantitative LCMSMS Utilizing native MSelectropherograms in proteomic analysis for discovering structure and interactioncorrelated differences

Coomassie staining provides routine (sub)femtomole in‐gel detection of intact proteoforms: Expanding opportunities for genuine Top‐down Proteomics

Published: Sep 26, 2017

Author(s): Nour Noaman, Prabhodh S. Abbineni, Michael Withers, Jens R. Coorssen

Journal: ELECTROPHORESIS

Abstract Modified colloidal Coomassie Brilliant Blue (cCBB) staining utilising a novel destain protocol and near‐infrared fluorescence detection (nIRFD) rivals the in‐gel protein detection sensitivity (DS) of SYPRO Ruby. However, established DS estimates are likely inaccurate in terms of 2DE‐resolved proteoform ‘spots’ since DS is routinely measured from comparatively diffuse protein ‘bands’ following wide‐well 1DE. Here, cCBB DS...

Read More thumbnail image: Coomassie staining provides routine subfemtomole ingel detection of intact proteoforms Expanding opportunities for genuine Topdown Proteomics

Cholesterol‐imprinted macroporous monoliths: Preparation and characterization

Published: Sep 26, 2017

Author(s): Mariia А. Stepanova, Lilia R. Kinziabulatova, Anna A. Nikitina, Evgenia G. Korzhikova‐Vlakh, Tatiana B. Tennikova

Journal: ELECTROPHORESIS

Abstract The development of sorbents for selective binding of cholesterol, which is a risk factor for cardiovascular disease, has a great importance for analytical science and medicine. In this work, two series of macroporous cholesterol‐imprinted monolithic sorbents differing in the composition of functional monomers (methacrylic acid, butyl methacrylate, 2‐hydroxyethyl methacrylate and ethylene dimethacrylate), amount of a...

Read More thumbnail image: Cholesterolimprinted macroporous monoliths Preparation and characterization

Sensitive phosphoprotein detection in SDS‐PAGE via Anthracene Chrome Red A stain

Published: Sep 26, 2017

Author(s): Sun‐Young Hwang, Jung‐Kap Choi

Journal: ELECTROPHORESIS

Abstract Protein phosphorylation, one of the most important post‐translational modifications, plays critical roles in many biological processes. Thus, it is necessary to precisely detect, identify and understand the phosphoproteins from protein mixture for the study of cell biology. We introduce a sensitive and specific detection method for phosphoproteins in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE)....

Read More thumbnail image: Sensitive phosphoprotein detection in SDSPAGE via Anthracene Chrome Red A stain

Quality evaluation of Guan‐Xin‐Ning injection based on fingerprint analysis and simultaneous separation and determination of seven bioactive constituents by capillary electrophoresis

Published: Sep 26, 2017

Author(s): Liying Xu, Ruimiao Chang, Meng Chen, Lou Li, Yayun Huang, Hongfen Zhang, Anjia Chen

Journal: ELECTROPHORESIS

Abstract The purpose of this study was to develop a comprehensive, rapid and practical capillary electrophoresis (CE) method for quality control (QC) of Guan‐Xin‐Ning (GXN) injection based on fingerprint analysis and simultaneous separation and determination of seven constituents. In fingerprint analysis, a capillary zone electrophoresis (CZE) method with a running buffer of 30 mM borate solution (pH 9.3) was established. Meanwhile,...

Read More thumbnail image: Quality evaluation of GuanXinNing injection based on fingerprint analysis and simultaneous separation and determination of seven bioactive constituents by capillary electrophoresis

Open‐tubular capillary electrochromatography using carboxylatopillar[5]arene as stationary phase

Published: Sep 26, 2017

Author(s): Deying Kong, Zilin Chen

Journal: ELECTROPHORESIS

Abstract Pillar[n]arenes have achieved much interest in material chemistry and supramolecular chemistry due to unusual pillar shape structure and high selectivity toward guest. However, pillar[n]arenes have not yet been applied in capillary electrochromatography. This work at first time reports that carboxylatopillar[5]arene is used as a stationary phase in open‐tubular capillary electrochromatography. Carboxylatopillar[5]arene not only...

Read More thumbnail image: Opentubular capillary electrochromatography using carboxylatopillar5arene as stationary phase

Advanced immunocapture of milk‐borne Salmonella by microfluidic magnetically stabilized fluidized bed

Published: Sep 26, 2017

Author(s): Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean‐Louis Viovy, Zuzana Bilkova

Journal: ELECTROPHORESIS

Abstract The success of microfluidic immunocapture based on magnetic beads depends primarily on a sophisticated microscale separation system and on the quality of the magnetic immunosorbent. A microfluidic chip containing a magnetically stabilized fluidized bed (μMSFB), developed for the capture and on‐chip amplification of bacteria, was recently described by Pereiro et al.. The present work shows the thorough development of...

Read More thumbnail image: Advanced immunocapture of milkborne Salmonella by microfluidic magnetically stabilized fluidized bed

Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract

Published: Sep 26, 2017

Author(s): Weili Huang, Xiaohui Guan, Yongchen Lv

Journal: Biomedical Chromatography

Abstract A specific and sensitive liquid chromatography tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the analysis of glaucocalyxin A and glaucocalyxin B in rat plasma using praeruptorin A as an internal standard. Separation was performed on a Hypurity C18 column (2.1 × 50 mm, 5 μm) with isocratic elution using 0.2% formic acid in water–acetonitrile (20:80, v/v). Mass spectrometric detection was...

Read More thumbnail image: Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LCMSMS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract

Simultaneous determination of boscalid and fludioxonil in grape and soil under field conditions by gas chromatography/tandem triple quadrupole mass spectrometry

Published: Sep 26, 2017

Author(s): Haizhen Zhang, A'wei Zhang, Min Huang, Weiwei Yu, Zhurui Li, Sizhuo Wu, Kunming Zheng, Kankan Zhang, Deyu Hu

Journal: Biomedical Chromatography

Abstract A gas chromatography–tandem mass spectrometry method was developed and validated to simultaneously determine boscalid and fludioxonil in grape and soil samples. These samples were extracted with 10 mL of acetonitrile and purified using a mixed primary secondary amine/octadecylsilane sorbent. The method showed good linearity (R2 > 0.99) in the calibration range 0.005–2 μg/mL for both pesticides. The limits of detection and...

Read More thumbnail image: Simultaneous determination of boscalid and fludioxonil in grape and soil under field conditions by gas chromatographytandem triple quadrupole mass spectrometry

LC‐ESI‐MS/MS determination of 4‐methylpyrazole in dog plasma and its application to a pharmacokinetic study in dogs

Published: Sep 25, 2017

Author(s): Devaraj V. Chandrasekhar, Ponnayyan Sulochana Suresh, Sreekanth Dittakavi, Rakesh A. Hiremath, Ravi Kanth Bhamidipati, Wolfgang Richter, Nuggehally R. Srinivas, Ramesh Mullangi

Journal: Biomedical Chromatography

Abstract A simple, specific, sensitive and rapid LC‐ESI‐MS/MS method has been developed and validated for the quantification of 4‐methylpyrazole in dog plasma using N‐methylnicotinamide‐d4 as an internal standard (IS) as per regulatory guidelines. Sample preparation was accomplished through a simple protein precipitation. Chromatographic separation of 4‐methylpyrazole and the IS was performed on a monolithic (Chromolith RP18e) column using an...

Read More thumbnail image: LCESIMSMS determination of 4methylpyrazole in dog plasma and its application to a pharmacokinetic study in dogs

Diffusion MRI fiber tractography of the brain

Published: Sep 25, 2017

Author(s): Ben Jeurissen, Maxime Descoteaux, Susumu Mori, Alexander Leemans

Journal: NMR in Biomedicine

The ability of fiber tractography to delineate non‐invasively the white matter fiber pathways of the brain raises possibilities for clinical applications and offers enormous potential for neuroscience. In the last decade, fiber tracking has become the method of choice to investigate quantitative MRI parameters in specific bundles of white matter. For neurosurgeons, it is quickly becoming an invaluable tool for the planning of surgery,...

Read More thumbnail image: Diffusion MRI fiber tractography of the brain

In vivo MRS for the assessment of mouse colon using a dedicated endorectal coil: initial findings

Published: Sep 25, 2017

Author(s): Hugo Dorez, Hélène Ratiney, Laurence Canaple, Hervé Saint‐Jalmes, Sophie Gaillard, Driffa Moussata, Raphaël Sablong, Olivier Beuf

Journal: NMR in Biomedicine

Inflammatory bowel disease is a common group of inflammation conditions that can affect the colon and the rectum. These pathologies require a careful follow‐up of patients to prevent the development of colorectal cancer. Currently, conventional endoscopy is used to depict alterations of the intestinal walls, and biopsies are performed on suspicious lesions for further analysis (histology). MRS enables the in vivo analysis of biochemical...

Read More thumbnail image: In vivo MRS for the assessment of mouse colon using a dedicated endorectal coil initial findings

Screening, separation, and evaluation of xanthine oxidase inhibitors from Paeonia lactiflora using chromatography combined with a multi‐mode microplate reader

Published: Sep 25, 2017

Author(s): Jing Wang, Dongfang Shi, Meizhu Zheng, Bing Ma, Jing Cui, Chunming Liu, Chengyu Liu

Journal: Journal of Separation Science

Abstract Natural products have become one of the most important resources for discovering novel xanthine oxidase inhibitors, which are commonly employed in the treatment of hyperuricemia and gout. However, to date, few reports exist regarding the use of monoterpene glycosides as xanthine oxidase inhibitors. Thus, we herein report the use of ultrafiltration coupled with liquid chromatography in the screening of monoterpene glycoside...

Read More thumbnail image: Screening separation and evaluation of xanthine oxidase inhibitors from Paeonia lactiflora using chromatography combined with a multimode microplate reader

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Published: Sep 25, 2017

Author(s): Václav Kašička

Journal: ELECTROPHORESIS

Abstract The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years...

Read More thumbnail image: Recent developments in capillary and microchip electroseparations of peptides 2015mid 2017

Controlled crosslinking of trimethylolpropane trimethacrylate for preparation of organic monolithic columns for capillary liquid chromatography

Published: Sep 25, 2017

Author(s): Mariana R. Gama, Pankaj Aggarwal, Milton L. Lee, Carla B.G. Bottoli

Journal: ELECTROPHORESIS

Abstract Organic monolithic columns based on single crosslinking of trimethylolpropane trimethacrylate (TRIM) monomer were prepared in a single step by living/controlled free‐radical polymerization. Full optimization of the preparation, such as using different percentages of TRIM and different amounts of radical promoter as well as various porogen solvents were explored. The resulting monolithic columns were characterized by...

Read More thumbnail image: Controlled crosslinking of trimethylolpropane trimethacrylate for preparation of organic monolithic columns for capillary liquid chromatography

An aptamer‐based paper microfluidic device for the colorimetric determination of cocaine

Published: Sep 25, 2017

Author(s): Ling Wang, Giacomo Musile, Bruce R. McCord

Journal: ELECTROPHORESIS

Abstract A method utilizing paper microfluidics coupled with gold nanoparticles and two anticocaine aptamers has been developed to detect seized cocaine samples. The ready‐to‐use format involves the use of a paper strip that produces a color change resulting from the salt‐induced aggregation of gold nanoparticles producing a visible color change indicating the presence of the drug. This format is specific for the detection of...

Read More thumbnail image: An aptamerbased paper microfluidic device for the colorimetric determination of cocaine

Simulation analysis of rectifying microfluidic mixing with field‐effect‐tunable electrothermal induced flow

Published: Sep 25, 2017

Author(s): Weiyu Liu, Yukun Ren, Ye Tao, Bobin Yao, You Li

Journal: ELECTROPHORESIS

Abstract We report herein field‐effect control on in‐phase electrothermal streaming from a theoretical point of view, a phenomenon termed “alternating‐current electrothermal‐flow field effect transistor” (ACET‐FFET), in the context of a new technology for handing analytes in microfluidics. Field‐effect control through a gate terminal endows ACET‐FFET the ability to generate arbitrary symmetry breaking in the transverse vortex flow...

Read More thumbnail image: Simulation analysis of rectifying microfluidic mixing with fieldeffecttunable electrothermal induced flow

A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

Published: Sep 25, 2017

Author(s): Ali Rahmani, Aliasghar Mohammadi, Hamid Reza Kalhor

Journal: ELECTROPHORESIS

Abstract In recent years, applications of dielectrophoresis‐based platforms have been recognized as effective and dependable approach to separate cells and bioparticles, suspended in different carrier fluids, based on particle size and electrical properties. In this study, a microfluidic device was fabricated by an unprecedented electrode pattern, and several experiments were performed to enrich samples including either of yeast,...

Read More thumbnail image: A continuous flow microfluidic device based on contactless dielectrophoresis for bioparticles enrichment

Recent advances in metal‐organic frameworks and covalent organic frameworks for sample preparation and chromatographic analysis

Published: Sep 25, 2017

Author(s): Xuan Wang, Nengsheng Ye

Journal: ELECTROPHORESIS

Abstract In the field of analytical chemistry, sample preparation and chromatographic separation are two core procedures. The means by which to improve the sensitivity, selectivity and detection limit of a method have become a topic of great interest. Recently, porous organic frameworks, such as metal‐organic frameworks (MOFs) and covalent organic frameworks (COFs), have been widely used in this research area because of their...

Read More thumbnail image: Recent advances in metalorganic frameworks and covalent organic frameworks for sample preparation and chromatographic analysis

Pencil graphite electrodes for improved electrochemical detection of oleuropein by the combination of Natural Deep Eutectic Solvents and graphene oxide

Published: Sep 25, 2017

Author(s): Federico J. V. Gomez#, Adrian Spisso, María Fernanda Silva

Journal: ELECTROPHORESIS

Abstract A novel methodology is presented for the enhanced electrochemical detection of oleuropein in complex plant matrices by Graphene Oxide Pencil Grahite Electrode (GOPGE) in combination with a buffer modified with a Natural Deep Eutectic Solvent, containing 10% (v/v) of Lactic acid, Glucose and H2O (LGH). The electrochemical behavior of oleuropein in the modified‐working buffer was examined using differential pulse voltammetry. ...

Read More thumbnail image: Pencil graphite electrodes for improved electrochemical detection of oleuropein by the combination of Natural Deep Eutectic Solvents and graphene oxide
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