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Simultaneous derivatization and air‐assisted liquid–liquid microextraction of some parabens in personal care products and their determination by GC with flame ionization detection
Published: Sep 25, 2013
Author(s): Mir Ali Farajzadeh, Elnaz Marzi Khosrowshahi, Parisa Khorram
Journal: Journal of Separation Science
A simultaneous derivatization/air‐assisted liquid–liquid microextraction technique has been developed for the sample pretreatment of some parabens in aqueous samples. The analytes were derivatized and extracted simultaneously by a fast reaction/extraction with butylchloroformate (derivatization agent/extraction solvent) from the aqueous samples and then analyzed by GC with flame ionization detection. The effect of catalyst type and...
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Electrolyte system strategies for anionic isotachophoresis with electrospray‐ionization mass‐spectrometric detection. 1. Regular isotachophoresis and free‐acid isotachophoresis.
Published: Sep 25, 2013
Author(s): Zdena Malá, Petr Gebauer, Petr Boček
Journal: ELECTROPHORESIS
The subject of this work is the definition of a simple model based on general ITP theory that allows describing and predicting the behavior of ITP systems compatible with ESI‐MS detection. The model is exemplified by anionic ITP of weak acids that represent an interesting potential application field of ITP‐ESI‐MS. Suitable ESI‐compatible electrolyte systems of very simple composition are proposed including a special free‐acid ITP...
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Differentiation of strains from the B acillus cereus group by RFLP ‐ PFGE genomic fingerprinting
Published: Sep 25, 2013
Author(s): Anna Otlewska, Elzbieta Oltuszak‐Walczak, Piotr Walczak
Journal: ELECTROPHORESIS
Bacillus mycoides, Bacillus pseudomycoides, Bacillus weihenstephanensis, Bacillus anthracis, Bacillus thuringiensis, and Bacillus cereus belong to the B. cereus group. The last three species are characterized by different phenotype features and pathogenicity spectrum, but it has been shown that these species are genetically closely related. The macrorestriction analysis of the genomic DNA with the NotI enzyme was used to generate polymorphism of restriction...
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Effects of fat on MR‐measured metabolite signal strengths: implications for in vivo MRS studies of the human brain
Published: Sep 24, 2013
Author(s): Anderson Mon, Christoph Abé, Timothy C. Durazzo, Dieter J. Meyerhoff
Journal: NMR in Biomedicine
Recent MRS studies have indicated that a higher body mass index (BMI) is associated with lower brain metabolite levels. Generally, individuals with higher BMIs have more body fat deposits than individuals with normal BMIs. This single‐voxel spectroscopy (SVS) study investigated possible effects of fat on MR‐measured metabolite signal areas, which may at least partly explain the observed associations of BMI with MR‐measured brain...
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Hyperpolarized 13 C NMR studies of glucose metabolism in living breast cancer cell cultures
Published: Sep 24, 2013
Author(s): T. Harris, H. Degani, L. Frydman
Journal: NMR in Biomedicine
The recent development of dissolution dynamic nuclear polarization (DNP) gives NMR the sensitivity to follow metabolic processes in living systems with high temporal resolution. In this article, we apply dissolution DNP to study the metabolism of hyperpolarized U‐13C,2H7‐glucose in living, perfused human breast cancer cells. Spectrally selective pulses were used to maximize the signal of the main product, lactate, whilst preserving the...
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Simultaneous isolation of seven compounds from G lehnia littoralis roots by off‐line overpressured layer chromatography guided by a TLC antioxidant autographic assay
Published: Sep 24, 2013
Author(s): Xing Su, Xiangkun Li, Hongxun Tao, Jinge Zhou, Tao Wu, Guixin Chou, Zhihong Cheng
Journal: Journal of Separation Science
A combinative method using overpressured layer chromatography (OPLC) and TLC bioautography against O2•− was developed to separate antioxidants from Glehnia littoralis roots. Through target‐directed isolation by the TLC bioautographic method, seven compounds including five antioxidants were rapidly isolated by OPLC and identified as 1‐linoloyl‐3‐palmitoylglycerol, facarindiol, panaxynol, isoimperatorin, β‐sitosterol, scopoletin, and umbelliferone from...
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Identification of annexin II as a novel secretory biomarker for breast cancer
Published: Sep 24, 2013
Author(s): You Rim Jeon, Sun Young Kim, Eun Jeong Lee, Yong Nyun Kim, Dong‐Young Noh, So Yeon Park, Aree Moon
Journal: PROTEOMICS
Early prediction of metastatic breast cancer is important for improvement of prognosis and survival rate. The present study aimed to identify secreted protein biomarkers for detection of invasive breast cancer. To this end, we performed a comparative proteomic analysis by a combination of 2DE and MALDI‐TOF MS analysis of conditioned media from invasive H‐Ras MCF10A human breast epithelial cells and noninvasive MCF10A and N‐Ras MCF10A...
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Development and validation of a fast and simple multi‐analyte procedure for quantification of 40 drugs relevant to emergency toxicology using GC‐MS and one‐point calibration
Published: Sep 24, 2013
Author(s): Golo M. J. Meyer, Armin A. Weber, Hans H. Maurer
Journal: Drug Testing and Analysis
Diagnosis and prognosis of poisonings should be confirmed by comprehensive screening and reliable quantification of xenobiotics, for example by gas chromatography–mass spectrometry (GC‐MS) or liquid chromatography‐mass spectrometry (LC‐MS). The turnaround time should be short enough to have an impact on clinical decisions. In emergency toxicology, quantification using full‐scan acquisition is preferable because this allows screening and...
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A population study of urine glycerol concentrations in elite athletes competing in North America
Published: Sep 24, 2013
Author(s): Brian N. Kelly, Myke Madsen, Ken Sharpe, Vinod Nair, Daniel Eichner
Journal: Drug Testing and Analysis
Glycerol is an endogenous substance that is on the World Anti‐Doping Agency's list of prohibited threshold substances due to its potential use as a plasma volume expansion agent. The WADA has set the threshold for urine glycerol, including measurement uncertainty, at 1.3 mg/mL. Glycerol in circulation largely comes from metabolism of triglycerides in order to meet energy requirements and when the renal threshold is eclipsed, glycerol is...
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Non‐enzymatic glycation and glycoxidation protein products in foods and diseases: An interconnected, complex scenario fully open to innovative proteomic studies
Published: Sep 24, 2013
Author(s): Simona Arena, Anna Maria Salzano, Giovanni Renzone, Chiara D'Ambrosio, Andrea Scaloni
Journal: Mass Spectrometry Reviews
Abstract The Maillard reaction includes a complex network of processes affecting food and biopharmaceutical products; it also occurs in living organisms and has been strictly related to cell aging, to the pathogenesis of several (chronic) diseases, such as diabetes, uremia, cataract, liver cirrhosis and various neurodegenerative pathologies, as well as to peritoneal dialysis treatment. Dozens of compounds are...
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Choice of optimal conditions for X‐ray fluorescence analysis of milk products with varying fat content
Published: Sep 23, 2013
Author(s): A. N. Smagunova, G. V. Pashkova
Journal: X-Ray Spectrometry
The design of experiments was used to study sources of errors in the sample preparation and to choose optimal conditions for X‐ray fluorescence (XRF) analysis of milk products of varying fat content. The measurements were performed using the conventional wavelength‐dispersive XRF (WDXRF) and the total reflection XRF (TXRF) techniques. For WDXRF, the dried milk samples were pelletized, and the mathematical models were constructed, which...
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Ultra‐high‐performance liquid chromatography–tandem mass spectrometry for pharmacokinetics of bakuchiol in rats
Published: Sep 23, 2013
Author(s): Shuang‐Qing Zhang, Yu‐Ming Fan
Journal: Biomedical Chromatography
ABSTRACT Psoralea Corylifolia L. is a traditional Chinese medicine with many beneficial effects in medical therapies. Bakuchiol was the main active ingredient of Psoralea Corylifolia L., used for the treatment of various diseases and also as a natural food additive. A specific and reliable ultra‐high performance liquid chromatography–tandem mass spectrometry has been developed and fully validated for the quantification of bakuchiol ...
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5‐(2‐Aminopropyl)indole (5‐IT): a psychoactive substance used for recreational purposes is an inhibitor of human monoamine oxidase (MAO)
Published: Sep 20, 2013
Author(s): Tomás Herraiz, Simon D. Brandt
Journal: Drug Testing and Analysis
5‐(2‐Aminopropyl)indole (5‐IT) is a psychoactive compound that has recently been associated with several fatal and non‐fatal intoxications in a number of European countries. There are indications that acute effects may include symptoms of monoaminergic (e.g. serotonin) toxicity and one mechanism involved in the increase of serotonin levels includes the inhibition of monoamine oxidase. This study investigated the effect of 5‐IT on human...
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Enantiomeric separations of illicit drugs and controlled substances using cyclofructan‐based (LARIHC) and cyclobond I 2000 RSP HPLC chiral stationary phases
Published: Sep 20, 2013
Author(s): Nilusha L.T. Padivitage, Edra Dodbiba, Zachary S. Breitbach, Daniel W. Armstrong
Journal: Drug Testing and Analysis
Recently a novel class of chiral stationary phases (CSPs) based on cyclofructan (CF) has been developed. Cyclofructans are cyclic oligosaccharides that possess a crown ether core and pendent fructofuranose moieties. Herein, we evaluate the applicability of these novel CSPs for the enantiomeric separation of chiral illicit drugs and controlled substances directly without any derivatization. A set of 20 racemic compounds were used to...
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Altitude exposure in sports: the Athlete Biological Passport standpoint
Published: Sep 20, 2013
Author(s): Fabian Sanchis‐Gomar, Helios Pareja Galeano, Thomas Brioche, Vladimir Martinez‐Bello, Giuseppe Lippi
Journal: Drug Testing and Analysis
The Athlete Biological Passport (ABP) is principally founded on monitoring an athlete's biological variables over time, to identify abnormal biases on a longitudinal basis. Several factors are known to influence the results of these markers. However, the manner in which the altitude factor is taken into account still needs to be standardized. Causal relationships between haematological variables should be correctly integrated into ABP...
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Noninvasive monitoring of photocatalytic degradation of X‐ray contrast media using Raman spectrometry
Published: Sep 20, 2013
Author(s): Sabina Salkic, Logan H. Eckler, Matthew J. Nee
Journal: Journal of Raman Spectroscopy
The photocatalytic degradation of the X‐ray contrast agents iohexol and diatrizoate are monitored by in situ Raman spectrometry measurements in aqueous solution with a TiO2 photocatalyst. Spectral features and changes are interpreted with the use of density functional theory calculations. While we observe similar results to those published previously, significant changes seen in the Raman spectrum allow us to better identify the mechanisms without...
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Preparation and evaluation of a novel molecularly imprinted SPE monolithic capillary column for the determination of auramine O in shrimp
Published: Sep 19, 2013
Author(s): Jiangmei Li, Haiyun Zhai, Zuanguang Chen, Qing Zhou, Zhenping Liu, Zihao Su
Journal: Journal of Separation Science
A novel method combining molecular imprinting and SPE was developed in a capillary column for the determination of auramine O in shrimp. The capillary monolithic column was prepared by UV‐initiated in situ polymerization, using auramine O as template and methacrylic acid and ethylene dimethacrylate as functional monomer and cross‐linker, respectively. The properties of the prepared capillary monolithic column were investigated under the optimized...
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Nanogaps in 2D Ag‐nanocap arrays for surface‐enhanced Raman scattering
Published: Sep 19, 2013
Author(s): Yongjun Zhang, Wenting Gao, Shuo Yang, Shanshan Liu, Xiaoyu Zhao, Ming Gao, Yaxin Wang, Jinghai Yang
Journal: Journal of Raman Spectroscopy
The surface‐enhanced Raman scattering substrate of Ag–Ag nanocap arrays are prepared by depositing Ag film onto two‐dimensional (2D) polystyrene colloidal nanosphere templates. When the original colloidal arrays are used as the substrate for Ag deposition, surface‐enhanced Raman scattering (SERS) enhancements show the strong size‐dependence behaviours. When O2‐plasma etched 2D polystyrene templates are used as the substrate for Ag deposition to form...
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Synthesis of uniform poly(d,l‐lactide) and poly(d,l‐lactide‐ co ‐glycolide) microspheres using a microfluidic chip for comparison
Published: Sep 19, 2013
Author(s): Chih‐Hui Yang, Keng‐Shiang Huang, Alexandru Mihai Grumezescu, Chih‐Yu Wang, Shian‐Chiuan Tzeng, Szu‐Yu Chen, Yu‐Hsin Lin, Yung‐Sheng Lin
Journal: ELECTROPHORESIS
Applications of poly(l‐lactide) (PLA) and poly(d,l‐lactide‐co‐glycolide) (PLGA) microspheres are widely used in the biomedical and pharmaceutical fields. The effects of PLA/PLGA on microsphere properties when using conventional particulate preparation methods are not easily defined due to the uncontrollable particle size and size distribution. This study was aimed to synthesize uniform PLA and PLGA microspheres using a phenol formaldehyde ...
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Multiplex genotyping assays for fine‐resolution subtyping of the major human Y ‐chromosome haplogroups E , G , I , J , and R in anthropological, genealogical, and forensic investigations
Published: Sep 19, 2013
Author(s): Mannis Oven, Kimberley Toscani, Nathalie Tempel, Arwin Ralf, Manfred Kayser
Journal: ELECTROPHORESIS
Inherited DNA polymorphisms located within the nonrecombing portion of the human Y chromosome provide a powerful means of tracking the patrilineal ancestry of male individuals. Recently, we introduced an efficient genotyping method for the detection of the basal Y‐chromosome haplogroups A to T, as well as an additional method for the dissection of haplogroup O into its sublineages. To further extend the use of the Y chromosome as an...
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