EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

Role of the mass attenuation coefficient database in standardization of a silicon drift X‐ray detector for PIXE analysis

Published: Sep 26, 2017

Author(s): Erin L. Flannigan, Christopher M. Heirwegh, John L. Campbell

Journal: X-Ray Spectrometry

A simple, inexpensive procedure is described for calibrating a silicon drift detector‐based PIXE system for the analysis of geological and other “thick‐target” materials. It rests on the use of single element, chemical compound, and National Institute for Standards and Technology multielement standards. Much less effort has been focussed on the impact of mass attenuation coefficients on PIXE's analytical accuracy than on ionization cross...

Read More thumbnail image: Role of the mass attenuation coefficient database in standardization of a silicon drift Xray detector for PIXE analysis

The effect of solvation processes on amino acid‐ and peptide‐silica stationary phases

Published: Sep 26, 2017

Author(s): Magdalena Skoczylas, Szymon Bocian, Tomasz Kowalkowski, Bogusław Buszewski

Journal: Journal of Separation Science

Abstract The surface excess adsorption isotherms of water, acetonitrile, and methanol from binary hydro‐organic mobile phases were investigated on nine home‐made stationary phases with chemically bonded amino acids, dipeptides, and tripeptides using the dynamic minor disturbance method. The stationary phases were modified by the following amino acids: glycine, alanine, phenylalanine, leucine, and aspartic acid. We investigated the...

Read More thumbnail image: The effect of solvation processes on amino acid and peptidesilica stationary phases

Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

Published: Sep 26, 2017

Author(s): Congcong Xu, Bing Wang, Yiqiong Pu, Jiansheng Tao, Tong Zhang

Journal: Journal of Separation Science

Abstract Triterpenes are a major class of chemical compounds found in natural plants and can be categorized into acyclic triterpenoids, monocyclic triterpenoids, tricyclic triterpenoids, tetracyclic triterpenoids, and pentacyclic triterpenoids. Among them, pentacyclic triterpenoids have gained more extensive attention due to their biological activities, including anti‐inflammation, antibacterial, antioxidation, antitumor, anti‐HIV,...

Read More thumbnail image: Techniques for the analysis of pentacyclic triterpenoids in medicinal plants

Rapid separation and simultaneous quantitative determination of 13 constituents in Psoraleae Fructus by a single marker using high‐performance liquid chromatography with diode array detection

Published: Sep 26, 2017

Author(s): Yimin Zhang, Zhiyong Chen, Xiaokun Xu, Qiang Zhou, Xiaolong Liu, Liping Liao, Zijia Zhang, Zhengtao Wang

Journal: Journal of Separation Science

Abstract Psoraleae Fructus is one of the most popular traditional Chinese medicines. Coumarins, flavonoids, and meroterpenes are the main contributors to the biological activity of Psoraleae Fructus. In this study, a new method for the quality control of Psoraleae Fructus was developed, through the quantitative analysis of multicomponents by single marker with diode array detector. Thirteen components, including psoralenoside,...

Read More thumbnail image: Rapid separation and simultaneous quantitative determination of 13 constituents in Psoraleae Fructus by a single marker using highperformance liquid chromatography with diode array detection

Enantioselective determination of 1‐[4‐(2‐methoxyethyl)phenoxy]‐3‐[2‐(2‐methoxyphenoxy)ethylamino]‐2‐propanol hydrochloride, a novel antihypertensive agent, in rat plasma and tissues by liquid chromatography–tandem mass spectrometry

Published: Sep 26, 2017

Author(s): Sijie He, Sanlan Wu, Wenwen Zhu, Yeli Guan, Gao Li, Luqin Si, Jiangeng Huang

Journal: Journal of Separation Science

Abstract Enantioselective biodistribution studies of 1‐[4‐(2‐methoxyethyl)phenoxy]‐3‐[2‐(2‐methoxyphenoxy)ethylamino]‐2‐propanol hydrochloride (TJ0711), a novel antihypertensive agent, require the accurate and precise quantification of each TJ0711 enantiomer in biological fluids and tissues. Here we report a simple and sensitive liquid chromatography with tandem mass spectrometry method for simultaneous determination of (R)‐TJ0711...

Read More thumbnail image: Enantioselective determination of 142methoxyethylphenoxy322methoxyphenoxyethylamino2propanol hydrochloride a novel antihypertensive agent in rat plasma and tissues by liquid chromatographytandem mass spectrometry

Application of dispersive liquid–liquid microextraction for the preconcentration of eight parabens in real samples and their determination by high‐performance liquid chromatography

Published: Sep 26, 2017

Author(s): Xiong Shen, Jian Liang, Luxia Zheng, Qianzhou Lv, Hong Wang

Journal: Journal of Separation Science

Abstract A simple and sensitive method for the simultaneous determination of eight parabens in human plasma and urine samples was developed. The samples were preconcentrated using dispersive liquid–liquid microextraction based on the solidification of floating organic drops and determined by high‐performance liquid chromatography with ultraviolet detection. The influence of variables affecting the extraction efficiency was...

Read More thumbnail image: Application of dispersive liquidliquid microextraction for the preconcentration of eight parabens in real samples and their determination by highperformance liquid chromatography

Stereoselective determination of dufulin in watermelon under field conditions using chiral ultra high performance liquid chromatography with high‐resolution mass spectrometry

Published: Sep 26, 2017

Author(s): Kunming Zheng, Jin Gong, Xiaoqin Li, Guifei Jia, Sizhuo Wu, Haizhen Zhang, Deyu Hu, Kankan Zhang

Journal: Journal of Separation Science

Abstract An effective and sensitive chiral analytical method was established to investigate the stereoselective dissipation of rac‐dufulin in watermelon using ultra high performance liquid chromatography with a superchiral S‐OD chiral column (4.6 × 150 mm i.d., 5 μm) coupled with high‐resolution mass spectrometry. To optimize the pretreatment method for detecting rac‐dufulin in the three matrixes, different extraction solvents,...

Read More thumbnail image: Stereoselective determination of dufulin in watermelon under field conditions using chiral ultra high performance liquid chromatography with highresolution mass spectrometry

Simultaneous determination of alcohols including diols and triols by HPLC with ultraviolet detection based on the formation of a copper(II) complex

Published: Sep 26, 2017

Author(s): Sohei Tanaka, Takumi Dohi, Sen‐ichi Aizawa, Tomoko Kemmei, Hiroyuki Terashima, Atsushi Taga, Atsushi Yamamoto, Shuji Kodama

Journal: Journal of Separation Science

Abstract We developed a reversed‐phase high‐performance liquid chromatography method with ultraviolet detection using on‐line complexation with Cu(II) ion for analysis of five alcohols including diols and triol (methanol, ethanol, 1,2‐propanediol, 1,3‐propanediol, and glycerol). The Cu(II) ion concentration in the mobile phase had a great influence on the peak areas of these alcohols, but not on their retention times. Column...

Read More thumbnail image: Simultaneous determination of alcohols including diols and triols by HPLC with ultraviolet detection based on the formation of a copperII complex

pH‐zone‐refining elution–extrusion countercurrent chromatography: Separation of hydroxyanthraquinones from Cassiae semen

Published: Sep 26, 2017

Author(s): Zhisi Bu, Liqiong Lv, Xingnuo Li, Chu Chu, Shengqiang Tong

Journal: Journal of Separation Science

Abstract Seven hydroxyanthraquinones were successfully separated from the traditional Chinese medicinal herb Cassiae semen by conventional and pH‐zone‐refining countercurrent chromatography with an environmentally friendly biphasic solvent system, in which elution–extrusion mode was investigated for pH‐zone‐refining countercurrent chromatography for the first time. A two‐phase solvent system composed of n‐hexane/ethyl acetate/ethanol/water...

Read More thumbnail image: pHzonerefining elutionextrusion countercurrent chromatography Separation of hydroxyanthraquinones from Cassiae semen

Development of multiple monolithic fiber solid‐phase microextraction and liquid chromatography–tandem mass spectrometry method for the sensitive monitoring of aminoglycosides in honey and milk samples

Published: Sep 26, 2017

Author(s): Lei Chen, Meng Mei, Xiaojia Huang

Journal: Journal of Separation Science

Abstract A simple and sensitive method for the simultaneous extraction and determination of six aminoglycosides in honey and milk samples was developed using multiple monolithic fiber solid‐phase microextraction and liquid chromatography with tandem mass spectrometry. The multiple monolithic fibers based on poly(methacrylic acid‐co‐ethylenedimethacrylate) monolith as the extraction medium was used to concentrate target analytes. Because there...

Read More thumbnail image: Development of multiple monolithic fiber solidphase microextraction and liquid chromatographytandem mass spectrometry method for the sensitive monitoring of aminoglycosides in honey and milk samples

Intermediate purification of CHO‐derived recombinant human Factor IX using hydrophobic interaction membrane‐based chromatography and its comparison to a sulfated resin

Published: Sep 26, 2017

Author(s): Daniel A. Ribeiro, Douglas F. Passos, Helen C. Ferraz, Leda R. Castilho

Journal: ELECTROPHORESIS

Abstract This work investigated the use of hydrophobic interaction membrane chromatography for intermediate purification of recombinant human Factor IX (rFIX) produced by CHO cells. The first purification step was based on a strong anion exchange monolith, thus forming a purification process fully based on convective media, which allow operation at high flow rates and low pressure drops, as well as modular scale‐up. Although the...

Read More thumbnail image: Intermediate purification of CHOderived recombinant human Factor IX using hydrophobic interaction membranebased chromatography and its comparison to a sulfated resin

A comparative analysis of human plasma and serum proteins by combining native PAGE, whole‐gel slicing and quantitative LC‐MS/MS: Utilizing native MS‐electropherograms in proteomic analysis for discovering structure and interaction‐correlated differences

Published: Sep 26, 2017

Author(s): Meiling Wen, Ya Jin, Takashi Manabe, Shumin Chen, Wen Tan

Journal: ELECTROPHORESIS

Abstract MS identification has long been used for PAGE‐separated protein bands, but global and systematic quantitation utilizing MS after PAGE has remained rare and not been reported for native PAGE. Here we reported on a new method combining native PAGE, whole‐gel slicing and quantitative LC‐MS/MS, aiming at comparative analysis on not only abundance, but also structures and interactions of proteins. A pair of human plasma and...

Read More thumbnail image: A comparative analysis of human plasma and serum proteins by combining native PAGE wholegel slicing and quantitative LCMSMS Utilizing native MSelectropherograms in proteomic analysis for discovering structure and interactioncorrelated differences

Coomassie staining provides routine (sub)femtomole in‐gel detection of intact proteoforms: Expanding opportunities for genuine Top‐down Proteomics

Published: Sep 26, 2017

Author(s): Nour Noaman, Prabhodh S. Abbineni, Michael Withers, Jens R. Coorssen

Journal: ELECTROPHORESIS

Abstract Modified colloidal Coomassie Brilliant Blue (cCBB) staining utilising a novel destain protocol and near‐infrared fluorescence detection (nIRFD) rivals the in‐gel protein detection sensitivity (DS) of SYPRO Ruby. However, established DS estimates are likely inaccurate in terms of 2DE‐resolved proteoform ‘spots’ since DS is routinely measured from comparatively diffuse protein ‘bands’ following wide‐well 1DE. Here, cCBB DS...

Read More thumbnail image: Coomassie staining provides routine subfemtomole ingel detection of intact proteoforms Expanding opportunities for genuine Topdown Proteomics

Cholesterol‐imprinted macroporous monoliths: Preparation and characterization

Published: Sep 26, 2017

Author(s): Mariia А. Stepanova, Lilia R. Kinziabulatova, Anna A. Nikitina, Evgenia G. Korzhikova‐Vlakh, Tatiana B. Tennikova

Journal: ELECTROPHORESIS

Abstract The development of sorbents for selective binding of cholesterol, which is a risk factor for cardiovascular disease, has a great importance for analytical science and medicine. In this work, two series of macroporous cholesterol‐imprinted monolithic sorbents differing in the composition of functional monomers (methacrylic acid, butyl methacrylate, 2‐hydroxyethyl methacrylate and ethylene dimethacrylate), amount of a...

Read More thumbnail image: Cholesterolimprinted macroporous monoliths Preparation and characterization

Sensitive phosphoprotein detection in SDS‐PAGE via Anthracene Chrome Red A stain

Published: Sep 26, 2017

Author(s): Sun‐Young Hwang, Jung‐Kap Choi

Journal: ELECTROPHORESIS

Abstract Protein phosphorylation, one of the most important post‐translational modifications, plays critical roles in many biological processes. Thus, it is necessary to precisely detect, identify and understand the phosphoproteins from protein mixture for the study of cell biology. We introduce a sensitive and specific detection method for phosphoproteins in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE)....

Read More thumbnail image: Sensitive phosphoprotein detection in SDSPAGE via Anthracene Chrome Red A stain

Quality evaluation of Guan‐Xin‐Ning injection based on fingerprint analysis and simultaneous separation and determination of seven bioactive constituents by capillary electrophoresis

Published: Sep 26, 2017

Author(s): Liying Xu, Ruimiao Chang, Meng Chen, Lou Li, Yayun Huang, Hongfen Zhang, Anjia Chen

Journal: ELECTROPHORESIS

Abstract The purpose of this study was to develop a comprehensive, rapid and practical capillary electrophoresis (CE) method for quality control (QC) of Guan‐Xin‐Ning (GXN) injection based on fingerprint analysis and simultaneous separation and determination of seven constituents. In fingerprint analysis, a capillary zone electrophoresis (CZE) method with a running buffer of 30 mM borate solution (pH 9.3) was established. Meanwhile,...

Read More thumbnail image: Quality evaluation of GuanXinNing injection based on fingerprint analysis and simultaneous separation and determination of seven bioactive constituents by capillary electrophoresis

Open‐tubular capillary electrochromatography using carboxylatopillar[5]arene as stationary phase

Published: Sep 26, 2017

Author(s): Deying Kong, Zilin Chen

Journal: ELECTROPHORESIS

Abstract Pillar[n]arenes have achieved much interest in material chemistry and supramolecular chemistry due to unusual pillar shape structure and high selectivity toward guest. However, pillar[n]arenes have not yet been applied in capillary electrochromatography. This work at first time reports that carboxylatopillar[5]arene is used as a stationary phase in open‐tubular capillary electrochromatography. Carboxylatopillar[5]arene not only...

Read More thumbnail image: Opentubular capillary electrochromatography using carboxylatopillar5arene as stationary phase

Advanced immunocapture of milk‐borne Salmonella by microfluidic magnetically stabilized fluidized bed

Published: Sep 26, 2017

Author(s): Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean‐Louis Viovy, Zuzana Bilkova

Journal: ELECTROPHORESIS

Abstract The success of microfluidic immunocapture based on magnetic beads depends primarily on a sophisticated microscale separation system and on the quality of the magnetic immunosorbent. A microfluidic chip containing a magnetically stabilized fluidized bed (μMSFB), developed for the capture and on‐chip amplification of bacteria, was recently described by Pereiro et al.. The present work shows the thorough development of...

Read More thumbnail image: Advanced immunocapture of milkborne Salmonella by microfluidic magnetically stabilized fluidized bed

Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract

Published: Sep 26, 2017

Author(s): Weili Huang, Xiaohui Guan, Yongchen Lv

Journal: Biomedical Chromatography

Abstract A specific and sensitive liquid chromatography tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the analysis of glaucocalyxin A and glaucocalyxin B in rat plasma using praeruptorin A as an internal standard. Separation was performed on a Hypurity C18 column (2.1 × 50 mm, 5 μm) with isocratic elution using 0.2% formic acid in water–acetonitrile (20:80, v/v). Mass spectrometric detection was...

Read More thumbnail image: Simultaneous determination of glaucocalyxin A and glaucocalyxin B in rat plasma by LCMSMS and its application to a pharmacokinetic study after oral administration of Rabdosia japonica extract

Simultaneous determination of boscalid and fludioxonil in grape and soil under field conditions by gas chromatography/tandem triple quadrupole mass spectrometry

Published: Sep 26, 2017

Author(s): Haizhen Zhang, A'wei Zhang, Min Huang, Weiwei Yu, Zhurui Li, Sizhuo Wu, Kunming Zheng, Kankan Zhang, Deyu Hu

Journal: Biomedical Chromatography

Abstract A gas chromatography–tandem mass spectrometry method was developed and validated to simultaneously determine boscalid and fludioxonil in grape and soil samples. These samples were extracted with 10 mL of acetonitrile and purified using a mixed primary secondary amine/octadecylsilane sorbent. The method showed good linearity (R2 > 0.99) in the calibration range 0.005–2 μg/mL for both pesticides. The limits of detection and...

Read More thumbnail image: Simultaneous determination of boscalid and fludioxonil in grape and soil under field conditions by gas chromatographytandem triple quadrupole mass spectrometry
Page:   Prev 2 3 4 5 6 7 8 9 10 11 12 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2017 John Wiley & Sons, Inc. All Rights Reserved