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Cytomorphology‐based microchip with contour extraction processing for bioparticle analysis

Published: Nov 26, 2018

Author(s): Yusheng Fu, Hong Ji

Journal: ELECTROPHORESIS

Abstract In this paper, we demonstrated an integrated digital image processing framework that is training‐free for high throughput beads or biological cells detection and enumeration by the bead aggregation splitting algorithm. By making contour extraction processing, the aggregated beads can be clearly split for precise counting. It can be potentially embedded on‐chip in a miniaturized medical equipment to automatically adjust...

Read More thumbnail image: Cytomorphologybased microchip with contour extraction processing for bioparticle analysis

Rapid quantification of quinine by multi‐stacking in a portable microchip electrophoresis system

Published: Nov 26, 2018

Author(s): Chee Tung Tai, Hong Heng See

Journal: ELECTROPHORESIS

Abstract A new multi‐stacking pre‐concentration procedure based on field‐enhanced sample injection (FESI), field‐amplified sample stacking, and transient isotachophoresis was developed and implemented in a compact microchip electrophoresis (MCE) with a double T‐junction glass chip, coupled with an on‐chip capacitively coupled contactless conductivity detection (C4D) system. A mixture of the cationic target analyte and the terminating...

Read More thumbnail image: Rapid quantification of quinine by multistacking in a portable microchip electrophoresis system

Urine protein quantification in stacking gel by SDS‐PAGE

Published: Nov 26, 2018

Author(s): Jia Jia, Jie Pan, Hongpan Xu, Sen Wang, Bing Bai

Journal: ELECTROPHORESIS

Abstract Urine total protein concentration is usually measured by the pyrogallol red‐molybdate (PRM) assay in clinical laboratories, but it is often subject to sample interference. Here, we introduce a stacking gel‐based method for accurate protein quantitation. In this method, the urine protein samples are run into the stacking gel by SDS‐PAGE where it is concentrated into a single band, and then quickly stained by 0.001% Coomassie...

Read More thumbnail image: Urine protein quantification in stacking gel by SDSPAGE

Development of a sensitive untargeted liquid chromatography–high resolution mass spectrometry screening devoted to hair analysis through a shared MS2 spectra database: A step toward early detection of new psychoactive substances

Published: Nov 25, 2018

Author(s): Nicolas Fabresse, Islam Amine Larabi, Tim Stratton, Robert Mistrik, Gregory Pfau, Geoffroy Lorin de la Grandmaison, Isabelle Etting, Stanislas Grassin Delyle, Jean‐Claude Alvarez

Journal: Drug Testing and Analysis

Abstract Untargeted liquid chromatography–high resolution mass spectrometry (LC–HRMS) techniques have become indispensable tools for systematic toxicological analysis. They allow the research of an almost unlimited number of drugs within a single analytical cycle, but shared mass spectra libraries are still missing to identify newly marketed compounds, along with defined analytical procedures. This article describes the...

Read More thumbnail image: Development of a sensitive untargeted liquid chromatographyhigh resolution mass spectrometry screening devoted to hair analysis through a shared MS2 spectra database A step toward early detection of new psychoactive substances

Glycoproteomic markers of hepatocellular carcinoma‐mass spectrometry based approaches

Published: Nov 25, 2018

Author(s): Jianhui Zhu, Elisa Warner, Neehar D. Parikh, David M. Lubman

Journal: Mass Spectrometry Reviews

Hepatocellular carcinoma (HCC) is the third most‐common cause of cancer‐related death worldwide. Most cases of HCC develop in patients that already have liver cirrhosis and have been recommended for surveillance for an early onset of HCC. Cirrhosis is the final common pathway for several etiologies of liver disease, including hepatitis B and C, alcohol, and increasingly non‐alcoholic fatty liver disease. Only 20–30%...

Read More thumbnail image: Glycoproteomic markers of hepatocellular carcinomamass spectrometry based approaches

A further insight into the metabolic profile of the nuclear receptor Rev‐erb agonist, SR9009

Published: Nov 22, 2018

Author(s): Monica Mazzarino, Nikla Rizzato, Carlotta Stacchini, Xavier Torre, Francesco Botrè

Journal: Drug Testing and Analysis

Abstract The reactions involved in the metabolic pathways of SR9009 were characterized by liquid chromatography–mass spectrometry (LC–MS) to identify the most appropriate marker(s) of use. The effects of gender, genetic polymorphism, and drug–drug interaction on the metabolic profile of SR9009 were also evaluated. In vitro approaches were based on the use of human liver microsomes and cytochrome P450 isoforms. Sample preparation...

Read More thumbnail image: A further insight into the metabolic profile of the nuclear receptor Reverb agonist SR9009

Zebrafish ( Danio rerio ) water tank model for the investigation of drug metabolism: Progress, outlook, and challenges

Published: Nov 22, 2018

Author(s): Vinicius Figueiredo Sardela, Carina de Souza Anselmo, Isabelle Karine da Costa Nunes, Gabriel Reis Alves Carneiro, Gustavo Ramalho Cardoso Santos, Aline Reis Carvalho, Bruna de Jesus Labanca, Daniely Silva Oliveira, William Dias Ribeiro, Amanda Lessa Dutra Araujo, Monica Costa Padilha, Cleverton Kleiton Freitas Lima, Valeria Pereira Sousa, Francisco Radler Aquino Neto, Henrique Marcelo Gualberto Pereira

Journal: Drug Testing and Analysis

Abstract Zebrafish (Danio rerio) water tank (ZWT) approach was investigated as an alternative model for metabolism studies based on six different experiments with four model compounds. Sibutramine was applied for the multivariate optimization of ZWT conditions, also for the comparison of the metabolism among ZWT, humans and mice, beyond for the role of CYP2B6 in ZWT. After the optimization, 18 fish and 168 hours of experiments...

Read More thumbnail image: Zebrafish  Danio rerio  water tank model for the investigation of drug metabolism Progress outlook and challenges

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Published: Nov 22, 2018

Author(s): Jianbo Li, Yue Jin, Huiyan Fu, Yihui Huang, Xianqin Wang, Yunfang Zhou

Journal: Biomedical Chromatography

Abstract Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti‐tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC–MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after...

Read More thumbnail image: Pharmacokinetics and bioavailability of gelsenicine in mice by UPLCMSMS

An air‐assisted flow‐gating interface for capillary electrophoresis

Published: Nov 22, 2018

Author(s): František Opekar, Petr Tůma

Journal: ELECTROPHORESIS

Abstract A new kind of flow gating interface (FGI) has been designed for online connection of CE with flow‐through analytical techniques. The sample is injected into the separation capillary from a space from which the BGE was forced out by compressed air. A drop of sample solution with a volume of 75 nL is formed between the outlet of the delivery capillary supplying the solution from the flow‐through apparatus and the entrance to...

Read More thumbnail image: An airassisted flowgating interface for capillary electrophoresis

Rapid separation of post‐blast explosive residues on glass electrophoresis microchips

Published: Nov 22, 2018

Author(s): Kemilly M. P. Pinheiro, Roger C. Moreira, Kariolanda C. A. Rezende, Márcio Talhavini, Lucio P. L. Logrado, José A. F. Baio, Marcos R. V. Lanza, Wendell K. T. Coltro

Journal: ELECTROPHORESIS

Abstract This study describes the development of an analytical methodology based on the use of microchip electrophoresis (ME) devices integrated with capacitively coupled contactless conductivity detection (C4D) for the separation and detection of inorganic anions in post‐blast explosive residues. The best separation condition was achieved using a running buffer composed of 35 mmol/L lactic acid, 10 mmol/L histidine and...

Read More thumbnail image: Rapid separation of postblast explosive residues on glass electrophoresis microchips

Metabolite profiling of l ‐isocorypalmine in rat urine, plasma, and feces after oral administration using high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry

Published: Nov 22, 2018

Author(s): Yiran Li, Teng Zhang, Jiaxin Huai, Congcong Cheng, Linlin Xie, Siqi Wang, Ronghua Dai

Journal: Journal of Separation Science

Abstract l‐Isocorypalmine, an active alkaloid compound isolated from Rhizoma Corydalis yanhusuo, has been reported to possess biological activity for treating cocaine use disorder. A high‐performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry method was established for identification of the metabolites of l‐isocorypalmine in urine, plasma and feces samples of rats after a single intragastric...

Read More thumbnail image: Metabolite profiling of l isocorypalmine in rat urine plasma and feces after oral administration using high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry

PLS multivariate analysis applied to corrosion studies on reinforced concrete

Published: Nov 22, 2018

Author(s): Pablo Monzón, José Enrique Ramón, José Manuel Gandía‐Romero, Manuel Valcuende, Juan Soto, Daniel Palací‐López

Journal: Journal of Chemometrics

Abstract There are few techniques available to calculate the corrosion rate (icorr) of reinforcing steel in concrete structures. This is due not only to a lack of instrumentation but also because it is necessary to take into account that polarization can irreversibly modify the metal surface and can affect the results or the future state of the metal. This is the reason some researchers prefer to test reinforcing steel with reversible...

Read More thumbnail image: PLS multivariate analysis applied to corrosion studies on reinforced concrete

Development of a headspace solid‐phase microextraction–surface‐enhanced Raman scattering approach to detect volatile pesticides

Published: Nov 22, 2018

Author(s): Chunrong Wang, Zhiyun Zhang, Lili He

Journal: Journal of Raman Spectroscopy

Abstract By coupling headspace solid‐phase microextraction (HS‐SPME) with surface‐enhanced Raman scattering (SERS), a facile and rapid method was developed for the detection of organophosphorus insecticides (phorate and isocarbophos) and organic sulfur bactericide (ferbam) in the vapour phase. Two types of SPME fibres based on stainless steel wires coated with gold (Au) nanoparticles were prepared. The fibre (Fibre II) prepared by...

Read More thumbnail image: Development of a headspace solidphase microextractionsurfaceenhanced Raman scattering approach to detect volatile pesticides

Raman spectra analysis of ZrO 2 thermally grown on Zircaloy substrates irradiated with heavy ion: Effects of oxygen isotopic substitution

Published: Nov 22, 2018

Author(s): Clément Ciszak, Michel Mermoux, Gaëlle Gutierrez, Frédéric Leprêtre, Christian Duriez, Ioana Popa, Laurent Fayette, Sébastien Chevalier

Journal: Journal of Raman Spectroscopy

Abstract Recently, unusual Raman signals were observed in different works conducted on thin zirconia layers grown on zirconium alloys simulating in‐reactor materials after high fluence ion irradiation or for samples cut from fuel rods irradiated in nuclear plants. As such, these spectra clearly do not correspond to any known spectrum of a pure standard zirconia phase. Therefore, the analysis conducted in this paper aims to provide a...

Read More thumbnail image: Raman spectra analysis of ZrO 2 thermally grown on Zircaloy substrates irradiated with heavy ion Effects of oxygen isotopic substitution

Machine learning algorithms applied to R aman spectra for the identification of variscite originating from the mining complex of G avà

Published: Nov 22, 2018

Author(s): José Francisco Díez‐Pastor, Susana Esther Jorge‐Villar, Álvar Arnaiz‐González, César Ignacio García‐Osorio, Yael Díaz‐Acha, Marc Campeny, Josep Bosch, Joan Carles Melgarejo

Journal: Journal of Raman Spectroscopy

Abstract Variscite is an aluminium phosphate mineral widely used as a gemstone in antiquity. Knowledge of the ancient trade in variscite has important implications on the historical appreciation of the commercial and migratory movements of human population. The mining complex of Gavà, which dates from the Neolithic, is one of the oldest underground mine sites in Europe, from where variscite was extracted from several mines and at...

Read More thumbnail image: Machine learning algorithms applied to R aman spectra for the identification of variscite originating from the mining complex of G ava

Determination of tubuloside B by LC–MS/MS and its application to a pharmacokinetic study in rats

Published: Nov 22, 2018

Author(s): Shengbao Yang, Ruiying Qu, Peilu Sun, Shan Xiong, Siyi Yan, Zhipeng Deng

Journal: Biomedical Chromatography

Read More thumbnail image: Determination of tubuloside B by LCMSMS and its application to a pharmacokinetic study in rats

Validated LC–MS/MS method for the quantification of sciadopitysin in rat plasma and its application to pharmacokinetic and bioavailability studies in vivo

Published: Nov 22, 2018

Author(s): Shengbao Yang, Ruiying Qu, Zhe Zhu, Wei Li, Chengliang Zhao, Liantai Li

Journal: Biomedical Chromatography

Read More thumbnail image: Validated LCMSMS method for the quantification of sciadopitysin in rat plasma and its application to pharmacokinetic and bioavailability studies in vivo

Rapid quantitative analysis of etoricoxib in human plasma by UPLC‐MS/MS and application to a pharmacokinetic study in Chinese healthy volunteers

Published: Nov 22, 2018

Author(s): Xiaoran Zhang, Nan Guo, Wei Ji, Qing Wen

Journal: Biomedical Chromatography

Abstract A selective and sensitive liquid chromatography–tandem mass spectrometry method was developed for simultaneous determination of etoricoxib in human plasma. Chromatography was performed on an Acquity UPLC HSS T3 column (1.8 μm, 50 × 2.1 mm), with a flow rate of 0.600 mL/min, using a gradient elution with acetonitrile and water which contained 2 mm ammonium acetate as the mobile phase. Detection was carried out on Triple QuadTM 5500 mass...

Read More thumbnail image: Rapid quantitative analysis of etoricoxib in human plasma by UPLCMSMS and application to a pharmacokinetic study in Chinese healthy volunteers

Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay

Published: Nov 21, 2018

Author(s): Kai Niu, Huiyu Yan, Chunjie Guo, Sixi Zhang, Wei Zhu, Shiyong Teng

Journal: Biomedical Chromatography

Abstract A highly sensitive LC–MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative‐ionization mass spectrometry in selected‐reaction monitoring mode. The assay was linear in the concentration range 4.52–904 ng/mL with intra‐ and...

Read More thumbnail image: Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LCMSMS assay

Target separation of flavonoids from Saxifraga tangutica using two‐dimensional hydrophilic interaction chromatography/reversed‐phase liquid chromatography

Published: Nov 21, 2018

Author(s): Jun Dang, Li Zhang, Qilan Wang, Lijuan Mei, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Yanduo Tao

Journal: Journal of Separation Science

Abstract An orthogonally (80.3%) preparative two‐dimensional hydrophilic interaction chromatography/reversed‐phase liquid chromatography method has been established for the isolation and purification of flavonoids from Saxifraga tangutica. Initially, flavonoids were enriched by means of a middle‐pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate...

Read More thumbnail image: Target separation of flavonoids from Saxifraga tangutica using twodimensional hydrophilic interaction chromatographyreversedphase liquid chromatography
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