UV-1900
Advantages of Low Stray Light

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  • Published: Jun 17, 2019
  • Source: Shimadzu Europa GmbH
  • Categories: UV/Vis Spectroscopy
thumbnail image: <font size=3>UV-1900</font><br />Advantages of Low Stray Light

UV-Visible spectroscopy is applied to a variety of standards and regulations such as pharmacopoeia or Restriction of Hazardous Substances (RoHS, Cr VI, https://www.shimadzu.eu/regulations). It is known that the concentration of a solution can be determined easily and precisely by a UV-Vis spectrophotometer. Recent demands for quantitative measurement by UV-Vis spectroscopy are the quantitation of a component with low concentration and the quantitation of a solution with high concentration without dilution. The quantitation limit and the linearity of a calibration curve are strongly related to the instrumental specifications such as stray light and photometric repeatability. The newly developed UV-1900 UV-VIS spectrophotometer uses the original Shimadzu “LO-RAY-LIGH®” grating to achieve low stray light with high photometric repeatability. Here, the quantitation limit and the linearity of a calibration curve for aqueous solutions of caffeine and acetic acid are obtained by using the UV-1900 as demonstrated.

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Measurement of Caffeine Aqueous Solution

The UV-1900 is a small-footprint spectrophotometer (450 (W) × 501 (D) × 244 (H) mm) in a hardware design based on ergonomics. The system uses a color touch panel (Fig. 2) as a control panel and features the easy-to-navigate user interface which shows the status and operating procedures at a glance. Furthermore, it has been awarded the Red Dot Design Award 2019 in the product design category.

Shimadzu UV-Vis Spectrophotometer UV-1900 with a scanning speed of up to 29.000 nm/min.

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Five standard caffeine aqueous solutions with different concentrations were prepared and measured using the conditions shown in Table 1. The UV-1900 has a function to capture a screen shot. Fig. 2 and Fig. 3 are screen shots showing their absorption spectra and a calibration curve obtained with their absorbance at 273 nm respectively. The calibration curve was expressed by Abs = 0.0528 Conc. and the square of the correlation coefficient stood at 0.9999.

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Fig. 1: Color Touch Display UV-1900.

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Fig. 2: Screen shot of Spectrum Measurement Spectra of Caffeine Aqueous Solution Concentrations from top to bottom: 40 mg/L, 20 mg/L, 10 mg/L, 4 mg/L and 2 mg/L.

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Fig.3 : Screen shot of Quantitation Measurement Calibration curve of Caffeine Aqueous Solutions.

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The quantitation limit is determined using the calibration curve and ten times the value of the standard deviation which is calculated with ten absorbance values of a blank solution. Table 2 shows ten absorbance values of water used as a blank solution at 273 nm and the resulting standard deviation σ. The quantitation limit of the caffeine aqueous solution which was calculated with 10 σ (0.00025 Abs) and the calibration curve (Abs = 0.0528 Conc.) was 0.005 mg/L. It is approximately ten times improved compared to the former model.

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Measurement of Acetic Acid Aqueous Solution

Six standard acetic acid aqueous solutions with different concentrations were prepared and measured using the conditions shown in Table 3. The UV-1900 can be operated not only in stand-alone mode but also by PC control using the accompanying Shimadzu UV software LabSolution UV. Fig. 4 and Fig. 5 show the absorption spectra measured and the calibration curve obtained with absorbance at 200 nm respectively. The calibration curve was expressed by Abs = 0.7111 Conc. and the square of the correlation coefficient was 0.9997. As the UV-1900 maintains low stray light even near 200 nm, a calibration curve with high linearity up to 2 Abs can be obtained.

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Fig. 4: Absorption Spectra of Acetic Acid Aqueous Solutions Black: 0.5 mg/mL, Red: 1.0 mg/mL, Blue: 1.5 mg/mL, Green: 2.0 mg/mL, Purple: 2.5 mg/mL, Orange: 3.0 mg/mL.

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Fig.5 : Calibration Curve of Acetic Acid Aqueous Solution.

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Conclusion

This report demonstrates that the Shimadzu UV-1900 achieving low stray light and high photometric repeatability enables the quantitative measurement of a solution with low concentration and the definition of a calibration curve with higher linearity. In combination with LabSolution DB or CS, UV-1900 can support FDA 21 CFR Part 11 as well as other guidelines.

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Shimadzu Europa GmbH

Duisburg, Germany

Phone: +49 203 76 87 0

shimadzu@shimadzu.eu
www.shimadzu.eu

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