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Separation of high‐purity eicosapentaenoic acid and docosahexaenoic acid from fish oil by pH‐zone‐refining countercurrent chromatography

Published: Jun 17, 2019

Author(s): Haoze Li, Zhen Yang, Xueli Cao, Tian Han, Hairun Pei

Journal: Journal of Separation Science

Abstract The evidence for unique effects of eicosapentaenoic acid and docosahexaenoic acid is growing. Further understanding and exploration of their independent effects in the nutraceutical and pharmaceutical industry is calling for the more efficient separation techniques to overcome the equivalent chain length rule of fatty acids. In this study, free eicosapentaenoic and docosahexaenoic acid were successfully separated by pH‐zone‐refining countercurrent chromatography for the first time....

Read More thumbnail image: Separation of highpurity eicosapentaenoic acid and docosahexaenoic acid from fish oil by pHzonerefining countercurrent chromatography

Optical tweezers combined with micro‐Raman investigation of alcohol‐induced changes on single, live red blood cells in blood plasma

Published: Jun 17, 2019

Author(s): Jijo Lukose, Mithun N, Ganesh Mohan, Shamee Shastry, Santhosh Chidangil

Journal: Journal of Raman Spectroscopy

Abstract Alcohol is commonly used as sterilizing agent in hospital settings. Alcohol abuse is also associated with numerous medical complications. It influences the coagulation mechanics and erythropoiesis. Optical tweezers are being extensively used in research labs for the trapping as well as manipulation of biological cells. The biochemical investigation of trapped biological cells can be realized with the integration of Raman spectroscopic technique with optical tweezers, which is termed...

Read More thumbnail image: Optical tweezers combined with microRaman investigation of alcoholinduced changes on single live red blood cells in blood plasma

An additional X‐ray diffraction by analyzer crystal

Published: Jun 17, 2019

Author(s): V.Ya. Borkhodoev

Journal: X-Ray Spectrometry

In the X‐ray spectra of samples from the boric acid and rock, obtained using an X‐ray spectrometer with a wave dispersive, an unknown emission line with a wavelength shorter than the short‐wavelength border of the bremsstrahlung of the X‐ray tube was detected. The intensity and position of the line in the spectrum depend on the voltage on the X‐ray tube. It has been established that this line appears as a result of X‐ray diffraction from the atomic planes of the analyzer crystal, which are...

Read More thumbnail image: An additional Xray diffraction by analyzer crystal

Elemental distribution by synchrotron X‐ray microfluorescence of prostate 3D cell culture

Published: Jun 17, 2019

Author(s): K.M.J. Rocha, R.G. Leitão, E.G. Oliveira‐Barros, M.A. Oliveira, C.G.L. Canellas, M.J. Anjos, L.E. Nasciutti, R.T. Lopes

Journal: X-Ray Spectrometry

Prostate cancer is a highly prevalent disease and ranks second among malignant neoplasms that affect men around the world, behind lung cancer alone. Trace elements are very important and are involved in many cellular processes. The X‐ray microfluorescence technique is an advanced tool of high spatial resolution, sensitivity, multielemental analysis, and nondestructiveness for trace element study. This study aimed to investigate the elemental distribution in spheroids obtained through the...

Read More thumbnail image: Elemental distribution by synchrotron Xray microfluorescence of prostate 3D cell culture

Measurements of L‐shell X‐ray emission lines of neonlike europium on an electron beam ion trap

Published: Jun 17, 2019

Author(s): Peter Beiersdorfer, Natalie Hell, Dmytro Panchenko, Greg V. Brown, Elmar Träbert, Richard L. Kelley, Caroline A. Kilbourne, F. Scott Porter

Journal: X-Ray Spectrometry

We report a measurement of the two X‐ray transitions that proceed from the and (1s22s1/22p63p1/2)J  =  1 upper levels to the (1s22s22p6)J  =  0 ground level in neonlike Eu53+ (Z = 63), that is,  near the previously documented avoided crossing of the two upper levels at Z = 68. The measurement was carried out using the calorimeter spectrometer on the Livermore EBIT‐I electron beam ion trap. It affirms the trends set by the neighboring neonlike ions both in terms of the relative intensity of the...

Read More thumbnail image: Measurements of Lshell Xray emission lines of neonlike europium on an electron beam ion trap

The scattering suppression of X‐rays with energy of 20–200 keV in spectrometers with Barkla polarizers

Published: Jun 17, 2019

Author(s): Batobolot Zhalsaraevich Zhalsaraev

Journal: X-Ray Spectrometry

The method is proposed for estimating the polarization parameters and the scattering suppression coefficients in energy dispersive X‐ray spectrometers with Barkla polarizers (EDPXRS) for X‐ray fluorescence (XRF) analysis. The scatter suppression coefficients are estimated accounting primary beam polarization, secondary beams yields, detectors response function, and partial factors. Estimations of scatter suppression coefficients due to polarization do not require the use of Stokes parameters...

Read More thumbnail image: The scattering suppression of Xrays with energy of 20200keV in spectrometers with Barkla polarizers

Separation of five diketopiperazines from the marine fungus Alternaria alternate HK‐25 by high‐speed counter‐current chromatography

Published: Jun 17, 2019

Author(s): Xiaoping He, Lijian Ding, Mengqi Yi, Jianzhou Xu, Xuezhen Zhou, Weiyan Zhang, Shan He

Journal: Journal of Separation Science

Abstract High‐speed counter‐current chromatography was applied to the separation of five diketoperazines from the marine Alternaria alternate HK‐25 for the first time using one‐step elution method with a pair of two‐phase solvent systems composed of petroleum ether/ethyl acetate/methanol/water (5.5:11:5:7, v/v). Where 151.6 mg of crude sample yielded five diketoperazines, 12,13‐dihydroxy‐fumitremorgin C (1), gliotoxin (2), demethoxyfum itremorgin C (3), bisdethiobis(methylthio)gliotoxin (4),...

Read More thumbnail image: Separation of five diketopiperazines from the marine fungus Alternaria alternate HK25 by highspeed countercurrent chromatography

Simultaneous quantification of urinary purines and creatinine by ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time‐of‐flight mass spectrometry: Method development, validation, and application to gout study

Published: Jun 17, 2019

Author(s): Yuan Yuan, Min Jiang, Hongyang Zhang, Ju Liu, Min Zhang, Ping Hu

Journal: Journal of Separation Science

Abstract We present an ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole time‐of‐flight mass spectrometry method for the simultaneous quantification of ten purines (adenine, hypoxanthine, guanine, xanthine, deoxyadenosine, adenosine, inosine, guanosine, xanthosine, and uric acid) and creatinine in human urine. After chromatographic separation on an ACE Excel 2 AQ column, high abundant creatinine and uric acid and the other low abundant purines were...

Read More thumbnail image: Simultaneous quantification of urinary purines and creatinine by ultra high performance liquid chromatography with ultraviolet spectroscopy and quadrupole timeofflight mass spectrometry Method development validation and application to gout study

Longitudinal preclinical magnetic resonance imaging of diffuse tumor burden in intramedullary myeloma following bortezomib therapy

Published: Jun 17, 2019

Author(s): Deep K. Hathi, John A. Engelbach, Jens Hillengass, Deborah Veis, Samuel Achilefu, Joel R. Garbow, Monica Shokeen

Journal: NMR in Biomedicine

Multiple myeloma (MM) is a largely incurable, debilitating hematologic malignancy of terminally differentiated plasma cells in the bone marrow (BM). Identification of therapeutic response is critical for improving outcomes and minimizing costs and off‐target toxicities. To assess changes in BM environmental factors and therapy efficacy, there is a need for noninvasive, nonionizing, longitudinal, preclinical methods. Here, we demonstrate the feasibility of preclinical magnetic resonance imaging...

Read More thumbnail image: Longitudinal preclinical magnetic resonance imaging of diffuse tumor burden in intramedullary myeloma following bortezomib therapy

Chemical profiling of the synthetic cannabinoid MDMB‐CHMICA: Identification, assessment, and stability study of synthesis‐related impurities in seized and synthesized samples

Published: Jun 17, 2019

Author(s): Sascha Münster‐Müller, Steven Hansen, Till Opatz, Ralf Zimmermann, Michael Pütz

Journal: Drug Testing and Analysis

Abstract In this work, the most discriminating synthesis‐related impurities found in samples from seizures and controlled synthesis of the synthetic cannabinoid MDMB‐CHMICA (methyl (S)‐2‐(1‐(cyclohexylmethyl)‐1H‐indole‐3‐carboxamido)‐3,3‐dimethylbutanoate) were characterized. Based on 61 available powder samples of MDMB‐CHMICA, 15 key‐impurities were assessed, isolated in larger quantities via flash chromatography and structurally elucidated and characterized via high resolution mass...

Read More thumbnail image: Chemical profiling of the synthetic cannabinoid MDMBCHMICA Identification assessment and stability study of synthesisrelated impurities in seized and synthesized samples

In vitro metabolism of the novel synthetic opioid agonist cyclopropylfentanyl and subsequent confirmation in authentic human samples using liquid chromatography‐high resolution mass spectrometry

Published: Jun 17, 2019

Author(s): Charlotte Cutler, Simon Hudson

Journal: Drug Testing and Analysis

Abstract Novel synthetic opioids (NSOs) are a class of novel psychoactive substances (NPS) that are growing in popularity and presenting a significant public health risk. Included in this class are derivatives of the highly potent analgesic, fentanyl. Cyclopropylfentanyl (CycP‐F) was first reported to the EU Early Warning System in August 2017, and was subsequently linked to more than 100 deaths in the US alone. Limited pharmacological, pharmacokinetic or toxicological data is available for...

Read More thumbnail image: In vitro metabolism of the novel synthetic opioid agonist cyclopropylfentanyl and subsequent confirmation in authentic human samples using liquid chromatographyhigh resolution mass spectrometry

Metabolic profiles and pharmacokinetics of goitrin in rats through liquid chromatography combined with electrospray ionization–tandem mass spectrometry

Published: Jun 17, 2019

Author(s): Jinhang Li, Yanhong Shi, Yan Xu, Li Yang, Zhengtao Wang, Han Han, Rui Wang

Journal: Biomedical Chromatography

Abstract Several chemical and biological studies have revealed R,S‐goitrin as the main bioactive constituent of Isatis indigotica Fort., responsible for antiviral antiendotoxin activity; however, few pharmacokinetic studies have been conducted. To comprehend the kinetics of R,S‐goitrin and promote its curative application, a rapid and sensitive UHPLC–MS/MS method was developed. The selected reaction monitoring transitions were m/z 130.0 → 70.0 for R,S‐goitrin and m/z 181.1 → 124.0 for the...

Read More thumbnail image: Metabolic profiles and pharmacokinetics of goitrin in rats through liquid chromatography combined with electrospray ionizationtandem mass spectrometry

The effects of electrostatic correlations on the ionic current rectification in conical nanopores

Published: Jun 17, 2019

Author(s): Elaheh Alidoosti, Hui Zhao

Journal: ELECTROPHORESIS

Abstract Ion‐ion electrostatic correlations are recognized to play a significant role in the presence of concentrated multivalent electrolytes. To account for their impact on ionic current rectification phenomenon in conical nanopores, we use the modified continuum Poisson‐Nernst‐Planck (PNP) equations by Bazant et al. Coupled with the Stokes equations, the effects of the EOF are also included. We thoroughly investigate the dependence of the ionic current rectification ratios as a function of...

Read More thumbnail image: The effects of electrostatic correlations on the ionic current rectification in conical nanopores

A new droplet breakup phenomenon in electrokinetic flow through a microchannel constriction

Published: Jun 14, 2019

Author(s): Xiang Ji, Teng Zhou, Yongbo Deng, Liuyong Shi, Xianman Zhang, Sang Woo Joo

Journal: ELECTROPHORESIS

Abstract A completely new droplet breakup phenomenon is reported for droplets passing through a constriction in an electrokinetic flow. The breakup occurs during the droplet shape recovery process past the constriction throat by the interplay of the dielectrophoretic stress release and the interface energy for droplets with smaller permittivity than that of the ambient fluid. There are conditions for constriction ratios and droplet size that the droplet breakup occurs. The numerical...

Read More thumbnail image: A new droplet breakup phenomenon in electrokinetic flow through a microchannel constriction

Simultaneous determination of 17 bisphenols in polycarbonate by ultra‐high performance supercritical fluid chromatography with tandem mass spectrometry

Published: Jun 14, 2019

Author(s): Yujia Zhang, Yun Ling, Yuan Zhang, Guihong Yao, Meiyi Yao, Feng Zhang

Journal: Journal of Separation Science

Abstract A novel method was developed for the first time for the determination of 17 bisphenols by ultra‐high performance supercritical fluid chromatography with tandem mass spectrometry. Under the optimal conditions, 17 bisphenols were separated successfully on a high density diol column in 9 min using methanol and carbon dioxide as mobile phase. 0.02% ammonium hydroxide/methanol v/v was used as the post‐column compensation solvent to improve response of mass spectrometry. Linear relations of...

Read More thumbnail image: Simultaneous determination of 17 bisphenols in polycarbonate by ultrahigh performance supercritical fluid chromatography with tandem mass spectrometry

Comparative pharmacokinetics and metabolites study of seven major bioactive components of Shaoyao‐Gancao decoction in normal and polycystic ovary syndrome rats by ultra high pressure liquid chromatography with tandem mass spectrometry

Published: Jun 14, 2019

Author(s): Jun‐jin Liu, Yao Cheng, Yun‐yun Shao, Zhuang‐peng Chang, Yi‐ting Guo, Xiao‐juan Feng, Ding Xu, Jing‐ping Zhang, Yan Song, Rui‐gang Hou

Journal: Journal of Separation Science

Abstract A simple and sensitive liquid chromatography with tandem mass spectrometry method was developed for simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, liquiritin, liquiritigenin, glycyrrhetinic acid, and glycyrrhizin in rat plasma after oral administration of Shaoyao‐Gancao decoction, which is traditionally used in the treatment of polycystic ovary syndrome. The plasma samples were pretreated with methanol as precipitant. The method exhibited good linearity...

Read More thumbnail image: Comparative pharmacokinetics and metabolites study of seven major bioactive components of ShaoyaoGancao decoction in normal and polycystic ovary syndrome rats by ultra high pressure liquid chromatography with tandem mass spectrometry

Combining Rapid Data Independent Acquisition and CRISPR Gene Deletion for Studying Potential Protein Functions: A Case of HMGN1

Published: Jun 14, 2019

Author(s): Martin Mehnert, Wenxue Li, Chongde Wu, Barbora Salovska, Yansheng Liu

Journal: PROTEOMICS

Abstract CRISPR‐Cas gene editing holds substantial promise in many biomedical disciplines and basic research. Due to the important functional implications of non‐histone chromosomal protein HMG‐14 (HMGN1) in regulating chromatin structure and tumor immunity, gene knockout of HMGN1 is performed by CRISPR in cancer cells and the following proteomic regulation events are studied. In particular, DIA mass spectrometry (DIA‐MS) is utilized, and more than 6200 proteins (protein‐ FDR 1%) and more than...

Read More thumbnail image: Combining Rapid Data Independent Acquisition and CRISPR Gene Deletion for Studying Potential Protein Functions A Case of HMGN1

Rapid detection of trace Cu2+ using an l‐cysteine based interdigitated electrode sensor integrated with AC electrokinetic enrichment

Published: Jun 14, 2019

Author(s): Haochen Qi, Meiqi Zhao, Huaguo Liang, Jayne Wu, Zhengfeng Huang, Anming Hu, Jian Wang, Yingchun Lu, Jian Zhang

Journal: ELECTROPHORESIS

Abstract Copper is an indispensable trace element for human health. Too much or too little intake of copper ion (Cu2+) can lead to its own adverse health conditions. Therefore, detection of Cu2+ is always of vital importance. In this work, a simple sensor was developed for rapid detection of trace Cu2+ in water, in which L‐cysteine (Cys) as a molecular probe was self‐assembled on a gold interdigital electrode to form a monolayer for specific capture of Cu2+. The interfacial capacitance of...

Read More thumbnail image: Rapid detection of trace Cu2 using an lcysteine based interdigitated electrode sensor integrated with AC electrokinetic enrichment

A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China

Published: Jun 13, 2019

Author(s): Shah Saud, Guoqing Li, Yingjie Sun, Muhammad Idrees Khan, Ashfaq Ur Rehman, Asad Uzzaman, Weiwen Liu, Chan Ding, Hua Xiao, Yuxing Wang, Chengxi Cao

Journal: ELECTROPHORESIS

Abstract A novel analytical protocol was developed for general quality screening of chicken meat based on IEF and protein extraction. To demonstrate the developed protocol, 24 chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water, and that in Group 3 were infected by new castle disease virus. Proteins were extracted from the meat samples by using pure water as an extractant, separated by IEF,...

Read More thumbnail image: A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China

Comparison of the peptidome released from keratins in Saiga antelope horn and goat horn under simulated gastrointestinal digestion

Published: Jun 13, 2019

Author(s): Rui Liu, Zhenhua Zhu, Dawei Qian, Jin‐ao Duan

Journal: ELECTROPHORESIS

Abstract Goat horn (Caprae Hircus Cornu, GH) has been used as a substitute for Saiga antelope horn (Saigae Tataricae Cornu, SAH) in the clinic and the pharmaceutical industry. In the present study, peptides released from SAH and GH under simulated gastric and intestinal digestion were identified. The results showed that most of the peptides released from SAH and GH under simulated gastrointestinal digestion were hydrophilic, and over 75% of the peptides from keratins (KRTs) were hydrophilic....

Read More thumbnail image: Comparison of the peptidome released from keratins in Saiga antelope horn and goat horn under simulated gastrointestinal digestion
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