EarlyView Articles

Skip to Navigation

The very latest articles from the scientific literature
on Wiley Online Library...


You can keep up-to date on these EarlyView articles, which focus on spectroscopy
and separation science:

Earlyview RSS Feed

rss

Filter by Journal

Browse By Journal

Discriminating polymorph distributions in pharmaceutical tablets using stimulated Raman scattering microscopy

Published: Oct 16, 2019

Author(s): Barbara Sarri, Rafaël Canonge, Xavier Audier, Valérie Lavastre, Géraldine Pénarier, Jean Alie, Hervé Rigneault

Journal: Journal of Raman Spectroscopy

Abstract Stimulated Raman scattering (SRS) imaging is used to probe active pharmaceutical ingredient exhibiting polymorphism within compact tablets. We show that few SRS images performed at selected wavenumbers allow to access both active pharmaceutical ingredients polymorphs and excipients from which molecular mapping is retrieved over millimetre‐size areas. We exemplified the approach using SRS images acquired at five wavenumbers to map clopidogrel and amibegron polymorphs embedded into...

Read More thumbnail image: Discriminating polymorph distributions in pharmaceutical tablets using stimulated Raman scattering microscopy

Theoretical simulation of surface‐enhanced resonance Raman spectroscopy of cytosine and its tautomers

Published: Oct 16, 2019

Author(s): Raziyeh Sharafdini, Mozhdeh Mohammadpour, Shapour Ramazani, Zahra Jamshidi

Journal: Journal of Raman Spectroscopy

Abstract The primary challenge of spectroscopic techniques in selective detection and characterization of tautomeric structures of DNA and RNA bases, and moreover, the accurate interpretation and explanation of the experimental results are the main motives of theoretical studies. Surface‐enhanced Raman spectroscopy (SERS) can be a powerful approach to distinguish cytosine in the presence of its tautomers. For this respect, herein, the theoretical simulation of the SERS spectra of cytosine and...

Read More thumbnail image: Theoretical simulation of surfaceenhanced resonance Raman spectroscopy of cytosine and its tautomers

Protein Paucimannosylation Is an Enriched N‐Glycosylation Signature of Human Cancers

Published: Oct 16, 2019

Author(s): Sayantani Chatterjee, Ling Y. Lee, Rebeca Kawahara, Jodie L. Abrahams, Barbara Adamczyk, Merrina Anugraham, Christopher Ashwood, Zeynep Sumer‐Bayraktar, Matthew T. Briggs, Jenny H. L. Chik, Arun Everest‐Dass, Sarah Förster, Hannes Hinneburg, Katia R. M. Leite, Ian Loke, Uwe Möginger, Edward S. X. Moh, Miyako Nakano, Saulo Recuero, Manveen K. Sethi, Miguel Srougi, Kathrin Stavenhagen, Vignesh Venkatakrishnan, Katherine Wongtrakul‐Kish, Simone Diestel, Peter Hoffmann, Niclas G. Karlsson, Daniel Kolarich, Mark P. Molloy, Michael H. Muders, Martin K. Oehler, Nicolle H. Packer, Giuseppe Palmisano, Morten Thaysen‐Andersen

Journal: PROTEOMICS

Abstract While aberrant protein glycosylation is a recognized characteristic of human cancers, advances in glycoanalytics continue to discover new associations between glycoproteins and tumorigenesis. This glycomics‐centric study investigates a possible link between protein paucimannosylation, an under‐studied class of human N‐glycosylation [Man1‐3GlcNAc2Fuc0‐1], and cancer. The paucimannosidic glycans (PMGs) of 34 cancer cell lines and 133 tissue samples spanning 11 cancer types and matching...

Read More thumbnail image: Protein Paucimannosylation Is an Enriched NGlycosylation Signature of Human Cancers

Automatic spike correction using UNIFIT 2020

Published: Oct 16, 2019

Author(s): Ronald Hesse, Carsten Bundesmann, Reinhard Denecke

Journal: Surface and Interface Analysis

The improvement of the software UNIFIT 2020 from an analysis processing software for photoelectron spectroscopy (XPS) only to a powerful tool for XPS, Auger electron spectroscopy (AES), X‐ray absorption spectroscopy (XAS), and Raman spectroscopy requires new additional programme routines. Particularly, the implementation of the analysis of Raman spectra needs a well‐working automatic spike correction. The application of the modified discrete Laplace operator method allows for a perfect...

Read More thumbnail image: Automatic spike correction using UNIFIT 2020

Impact of moderate pump–Stokes chirp on femtosecond coherent anti‐Stokes Raman scattering spectra

Published: Oct 16, 2019

Author(s): Mingming Gu, Aman Satija, Robert P. Lucht

Journal: Journal of Raman Spectroscopy

Abstract The effects of inducing moderate chirp in the pump and Stokes beams in chirped‐probe‐pulse femtosecond coherent anti‐Stokes Raman scattering (CARS) spectroscopy are discussed. This is an important issue for measurements in high‐pressure systems where the CARS beams must be transmitted through glass of significant thickness to reach the probe volume of interest. The effects were investigated both experimentally, by inserting disks of SF‐11 glass into the pump and Stokes beam paths, and...

Read More thumbnail image: Impact of moderate pumpStokes chirp on femtosecond coherent antiStokes Raman scattering spectra

STRATEGIES AND CHALLENGES IN METHOD DEVELOPMENT AND VALIDATION FOR THE ABSOLUTE QUANTIFICATION OF ENDOGENOUS BIOMARKER METABOLITES USING LIQUID CHROMATOGRAPHY‐TANDEM MASS SPECTROMETRY

Published: Oct 15, 2019

Author(s): Mona M. Khamis, Darryl J. Adamko, Anas El‐Aneed

Journal: Mass Spectrometry Reviews

Metabolomics is a dynamically evolving field, with a major application in identifying biomarkers for drug development and personalized medicine. Numerous metabolomic studies have identified endogenous metabolites that, in principle, are eligible for translation to clinical practice. However, few metabolomic‐derived biomarker candidates have been qualified by regulatory bodies for clinical applications. Such interruption in the biomarker qualification process can be largely attributed to...

Read More thumbnail image: STRATEGIES AND CHALLENGES IN METHOD DEVELOPMENT AND VALIDATION FOR THE ABSOLUTE QUANTIFICATION OF ENDOGENOUS BIOMARKER METABOLITES USING LIQUID CHROMATOGRAPHYTANDEM MASS SPECTROMETRY

Proteome Oxidative Modifications and Impairment of Specific Metabolic Pathways During Cellular Senescence and Aging

Published: Oct 15, 2019

Author(s): Marie‐Paule Hamon, Emad K. Ahmed, Martín A. Baraibar, Bertrand Friguet

Journal: PROTEOMICS

Abstract Accumulation of oxidatively modified proteins is a hallmark of organismal aging in vivo and of cellular replicative senescence in vitro. Failure of protein maintenance is a major contributor to the age‐associated accumulation of damaged proteins that is believed to participate to the age‐related decline in cellular function. In this context, quantitative proteomics approaches, including 2‐D gel electrophoresis (2‐DE)‐based methods, represent powerful tools for monitoring the extent of...

Read More thumbnail image: Proteome Oxidative Modifications and Impairment of Specific Metabolic Pathways During Cellular Senescence and Aging

Recent developments in DNA adduct analysis using liquid chromatography coupled with mass spectrometry

Published: Oct 15, 2019

Author(s): Yu Tang, Jin‐Lan Zhang

Journal: Journal of Separation Science

Abstract The formation of DNA adducts by genotoxic agents is an early event in cancer development, and it may lead to gene mutations, thereby initiating tumor development. The measurement of DNA adducts can provide critical information about the genotoxic potential of a chemical and its mechanism of carcinogenesis. In recent decades, liquid chromatography coupled with mass spectrometry has become the most important technique for analyzing DNA adducts. The improvements in resolution achievable...

Read More thumbnail image: Recent developments in DNA adduct analysis using liquid chromatography coupled with mass spectrometry

Probing the degradation mechanism of forsythiaside A and simultaneous determination of three forsythiasides in Forsythia preparations by a single marker

Published: Oct 15, 2019

Author(s): Minghui Qi, Shiyi Zhao, Bin Zhou, Min Zhang, Hongyang Zhang, Yuerong Wang, Ping Hu

Journal: Journal of Separation Science

Abstract Forsythiaside A is the major component of Forsythia suspensa. This study investigated the degradation mechanism of forsythiaside A. Eight degraded components including forsythiaside I, forsythiaside H, forsythiaside E, caffeic acid, suspensaside A, β‐hydroxy forsythiaside I, β‐hydroxy forsythiaside H, and β‐hydroxy forsythiaside A were identified by using ultra‐high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry. Then, the quantitative analysis of...

Read More thumbnail image: Probing the degradation mechanism of forsythiaside A and simultaneous determination of three forsythiasides in Forsythia preparations by a single marker

Preparation of chitosan‐based molecularly imprinted material for enantioseparation of racemic mandelic acid in aqueous medium by solid phase extraction

Published: Oct 15, 2019

Author(s): Xudong Xiao, Zhenqun Li, Ya Liu, Li Jia

Journal: Journal of Separation Science

Abstract An S‐mandelic acid imprinted chitosan resin was synthesized by cross‐linking chitosan with glutaraldehyde in 2% acetic acid solution. S‐Mandelic acid imprinted chitosan resin was used to enantioselectively separate racemic mandelic acid in aqueous medium. When keeping the pH of sample solution (100 mM Tris‐H3PO4) at 3.5 and adsorption time at 40 min, the enantiomer excess of mandelic acid in supernatant was 78.8%. The adsorption capacities of S‐mandelic acid imprinted chitosan resin...

Read More thumbnail image: Preparation of chitosanbased molecularly imprinted material for enantioseparation of racemic mandelic acid in aqueous medium by solid phase extraction

In‐situ photopolymerized polyhedral oligomeric silsesquioxane‐derived monolithic capillary columns with quinidine functionality for enantioseparation by nano‐liquid chromatography

Published: Oct 15, 2019

Author(s): Siyao Liu, Xinyue Fu, Ronny Löffler, Michael Lämmerhofer

Journal: ELECTROPHORESIS

Abstract The successful fabrication of monolithic capillary columns for enantiomer separations was achieved within vinylized fused silica capillaries via fast “one‐pot” photo‐initiated free radical polymerization reaction. A mixture consisting of polyhedral oligomeric silsesquioxane, O‐[2‐(methacryloyloxy)ethylcarbamoyl]‐10,11‐dihydroquinidine was copolymerized in the presence of n‐butanol, ethylene glycol and photo‐initiator 2,2‐dimethoxy‐2‐phenylacetophenone. The morphology of the resultant...

Read More thumbnail image: Insitu photopolymerized polyhedral oligomeric silsesquioxanederived monolithic capillary columns with quinidine functionality for enantioseparation by nanoliquid chromatography

Analytical tools for monitoring changes in physical and chemical properties of chromatography resin upon reuse

Published: Oct 15, 2019

Author(s): Mili Pathak, Katherine Lintern, Thomas F. Johnson, Aswathy M. Nair, Soumyo Mukherji, Daniel G. Bracewell, Anurag S. Rathore

Journal: ELECTROPHORESIS

Abstract Protein A resins are often reused for multiple cycles to improve process economy during mAb purification. Significant reduction in binding capacity and product recovery are typically observed due to the presence of unwanted materials (foulants) deposited on the resin upon reuse. In this paper, we have used a wide spectrum of qualitative and quantitative analytical tools (particle size analysis, HPLC, fluorescence, SEM, MS, and FTIR) to compare the strengths and shortcomings of...

Read More thumbnail image: Analytical tools for monitoring changes in physical and chemical properties of chromatography resin upon reuse

Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI) for Monitoring of Drug Response in Primary Cancer Spheroids

Published: Oct 15, 2019

Author(s): Peter Hoffmann, Manuela Klingler‐Hoffmann, Martin K Oehler, Carmela Ricciardelli, Noor A Lokman, Zoe K Price, Parul Mittal

Journal: PROTEOMICS

Abstract Malignant ascites is a fluid, which builds up in the abdomen and contains cancer cells in the form of single cells or multicellular clusters called spheroids. Malignant ascites has been observed in patients suffering from ovarian, cervical, gastric, colorectal, pancreatic, endometrial, or primary liver cancer. The spheroids are believed to play a major role in chemo resistance and metastasis of the cancer. To ease the discomfort of patients, malignant ascites (MA) is often drained...

Read More thumbnail image: Matrix Assisted Laser DesorptionIonization Mass Spectrometry Imaging MALDI MSI for Monitoring of Drug Response in Primary Cancer Spheroids

A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm

Published: Oct 14, 2019

Author(s): Sumit Kumar Singh, Garvit Goel, Anurag S. Rathore

Journal: ELECTROPHORESIS

Abstract A unique peptide based search algorithm for identification of protein mixture using PMF is proposed. The proposed search algorithm utilizes binary search and heapsort programs to generate frequency chart depicting the unique peptides corresponding to all proteins in a proteome. The use of binary search program significantly reduces the time for frequency chart preparation to ∼2 s for a proteome comprising ∼23 000 proteins. The algorithm was applied to a three‐protein mixture...

Read More thumbnail image: A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algorithm

Evaluating the feasibility of creatine‐weighted CEST MRI in human brain at 7 T using a Z‐spectral fitting approach

Published: Oct 14, 2019

Author(s): Anup Singh, Ayan Debnath, Kejia Cai, Puneet Bagga, Mohammad Haris, Hari Hariharan, Ravinder Reddy

Journal: NMR in Biomedicine

The current study aims to evaluate the feasibility of creatine (Cr) chemical exchange saturation transfer (CEST)‐weighted MRI at 7 T in the human brain by optimizing the saturation pulse parameters and computing contrast using a Z‐spectral fitting approach. The Cr‐weighted (Cr‐w) CEST contrast was computed from phantoms data. Simulations were carried out to obtain the optimum saturation parameters for Cr‐w CEST with lower contribution from other brain metabolites. CEST‐w images were acquired...

Read More thumbnail image: Evaluating the feasibility of creatineweighted CEST MRI in human brain at 7 T using a Zspectral fitting approach

Low SAR 31P (multi‐echo) spectroscopic imaging using an integrated whole‐body transmit coil at 7T

Published: Oct 14, 2019

Author(s): Q. Houtum, D. Welting, W.J.M. Gosselink, D.W.J. Klomp, C.S. Arteaga de Castro, W.J.M. Kemp

Journal: NMR in Biomedicine

Phosphorus (31P) MRSI provides opportunities to monitor potential biomarkers. However, current applications of 31P MRS are generally restricted to relatively small volumes as small coils are used. Conventional surface coils require high energy adiabatic RF pulses to achieve flip angle homogeneity, leading to high specific absorption rates (SARs), and occupy space within the MRI bore. A birdcage coil behind the bore cover can potentially reduce the SAR constraints massively by use of...

Read More thumbnail image: Low SAR 31P multiecho spectroscopic imaging using an integrated wholebody transmit coil at 7T

Characterization of in vitro neural functional connectivity on a neurofluidic device

Published: Oct 14, 2019

Author(s): Xuefei Shen, Jiaxi Wu, Zhengfei Wang, Tao Chen

Journal: ELECTROPHORESIS

Abstract Understanding the mechanism of functional connectivity in neural system is of great benefit to lot of researches and applications. Microfluidics and microelectrode arrays (MEAs) have been frequently utilized for in vitro neural cultures study. However, there are few studies on the functional connectivity of neural cultures grown on a microfluidic chip. It is intriguing to unveil the influences of microfluidic structures on in vitro neuronal networks from the perspective of functional...

Read More thumbnail image: Characterization of in vitro neural functional connectivity on a neurofluidic device

Development of an Analytical Method for the Metaproteomic Investigation of Bioaerosol from Work Environments

Published: Oct 14, 2019

Author(s): Susy Piovesana, Anna Laura Capriotti, Patrizia Foglia, Carmela Maria Montone, Giorgia La Barbera, Riccardo Zenezini Chiozzi, Aldo Laganà, Chiara Cavaliere

Journal: PROTEOMICS

Abstract The metaproteomic analysis of air particulate matter provides valuable information about the properties of bioaerosols in the atmosphere and their influence on climate and public health. In this work, a new method for the extraction and analysis of proteins in airborne particulate matter from quartz microfiber filters is developed. Different protein extraction procedures are tested to select the best extraction protocol based on protein recovery. The optimized method is tested for the...

Read More thumbnail image: Development of an Analytical Method for the Metaproteomic Investigation of Bioaerosol from Work Environments

3D aluminum/silver hierarchical nanostructure with large areas of dense hot spots for surface‐enhanced raman scattering

Published: Oct 14, 2019

Author(s): Nan Zhao, Hefu Li, Yanru xie, Zhenbao Feng, Zongliang Wang, Zhenshan Yang, Xunling Yan, Wenjun Wang, Cunwei Tian, Huishan Yu

Journal: ELECTROPHORESIS

Abstract Plasmonic nanomaterials possessing large‐volume, high‐density hot spots with high field enhancement are highly desirable for ultrasensitive surface‐enhanced Raman scattering (SERS) sensing. However, many as‐prepared plasmonic nanomaterials are limited in available dense hot spots and in sample size, which greatly hinder their wide applications in SERS devices. Here, we develop a two‐step physical deposition protocol and successfully fabricate 3D hierarchical nanostructures with highly...

Read More thumbnail image: 3D aluminumsilver hierarchical nanostructure with large areas of dense hot spots for surfaceenhanced raman scattering

Unsupervised machine learning for exploratory data analysis in imaging mass spectrometry

Published: Oct 11, 2019

Author(s): Nico Verbeeck, Richard M. Caprioli, Raf Van de Plas

Journal: Mass Spectrometry Reviews

Imaging mass spectrometry (IMS) is a rapidly advancing molecular imaging modality that can map the spatial distribution of molecules with high chemical specificity. IMS does not require prior tagging of molecular targets and is able to measure a large number of ions concurrently in a single experiment. While this makes it particularly suited for exploratory analysis, the large amount and high‐dimensional nature of data generated by IMS techniques make automated computational analysis...

Read More thumbnail image: Unsupervised machine learning for exploratory data analysis in imaging mass spectrometry
Page:   1 2 3 4 5 6 Next

Social Links

Share This Links

Bookmark and Share

Microsites

Suppliers Selection
Societies Selection

Banner Ad

Click here to see
all job opportunities

Most Viewed

Copyright Information

Interested in separation science? Visit our sister site separationsNOW.com

Copyright © 2019 John Wiley & Sons, Inc. All Rights Reserved