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The Nucleotide‐Dependent Interactome of Rice Heterotrimeric G‐Protein α ‐Subunit

Published: Apr 18, 2019

Author(s): Akshaya Kumar Biswal, Evan Wesley McConnell, Emily Grace Werth, Shuen‐Fang Lo, Su‐May Yu, Leslie M. Hicks, Alan M. Jones

Journal: PROTEOMICS

Abstract The rice heterotrimeric G‐protein complex, a guanine‐nucleotide‐dependent on‐off switch, mediates vital cellular processes and responses to biotic and abiotic stress. Exchange of bound GDP (resting state) for GTP (active state) is spontaneous in plants including rice and thus there is no need for promoting guanine nucleotide exchange in vivo as a mechanism for regulating the active state of signaling as it is well known for animal G signaling. As such, a master regulator controlling...

Read More thumbnail image: The NucleotideDependent Interactome of Rice Heterotrimeric GProtein  Subunit

Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment

Published: Apr 18, 2019

Author(s): Yan Gao, Hyojin Lee, Oh Kwang Kwon, Zhongyi Cheng, Minjia Tan, Ki‐Tae Kim, Sangkyu Lee

Journal: PROTEOMICS

Abstract Histidine phosphorylation is a reversible post‐translational modification that is known to regulate signal transduction in prokaryotes. However, functional studies in eukaryotes have been largely neglected due to the labile nature of N‐linked phosphorylated amino acids. In an effort to help elucidate the heretofore hidden vertebrate phosphoproteome, this report presents a global phosphorylation analysis of Danio rerio (zebrafish) larvae. Phosphopeptide enrichment is performed using a...

Read More thumbnail image: Profiling of Histidine Phosphoproteome in Danio rerio by TiO2 Enrichment

A Fast and Economical Sample Preparation Protocol for Interaction Proteomics Analysis

Published: Apr 18, 2019

Author(s): Miguel A. Gonzalez‐Lozano, Frank Koopmans, Iryna Paliukhovich, August B. Smit, Ka Wan Li

Journal: PROTEOMICS

Abstract A simple and fast immunoprecipitation (IP) protocol is designed with the sample preparation incorporated, applicable to both low and high throughput. This new protocol combines two procedures based on magnetic beads in 96‐well plate format. Protein complexes are captured by antibodies and magnetic beads conjugated with protein A. Proteins are washed and on‐bead digested by using Single‐Pot solid‐phase sample preparation (SP3). The whole IP‐SP3 approach can be completed in one day,...

Read More thumbnail image: A Fast and Economical Sample Preparation Protocol for Interaction Proteomics Analysis

Characteristics of aerosol pollution in the vicinity of an oil refinery near Rijeka, Croatia

Published: Apr 18, 2019

Author(s): Marija Čargonja, Darko Mekterović, Diana Mance, Gordana Žauhar, Iva Bogdanović Radović, Ivana Zamboni

Journal: X-Ray Spectrometry

Fine aerosol pollution is one of the most important factors in air pollution monitoring. Industrial production often represents significant source of aerosol pollution in surrounding areas, and therefore, it is important to understand its impact on air quality. In order to investigate contribution coming from the oil refinery to air pollution, PM2.5 (particulate matter with aerodynamic diameter smaller than 2.5 μm) samples were collected in the vicinity of an oil refinery during the 1‐year...

Read More thumbnail image: Characteristics of aerosol pollution in the vicinity of an oil refinery near Rijeka Croatia

Review of highly charged ion production with ECR ion source and the future opportunities for HCI physics

Published: Apr 18, 2019

Author(s): L. Sun, H.W. Zhao, W. Lu, J.W. Guo, Y. Yang, H. Jia, L. Lu, W. Wu

Journal: X-Ray Spectrometry

In recent years, there is very intense worldwide research and development work on electron cyclotron resonance ion source (ECRIS). Remarkable progress represented by the third generation superconducting ECRIS has been made with regards of intense highly charged ion beam production such as 600 eμA Ar16+, 10 eμA Ar18+, and hundreds of enA He‐like Kr34+. A low energy heavy ion platform named Low Energy heavy ion Accelerator Facility (LEAF) that features a next generation 45 GHz ECRIS, a 300 kV...

Read More thumbnail image: Review of highly charged ion production with ECR ion source and the future opportunities for HCI physics

Quantitative determination of macroelement and microelement content of fresh and processed bamboo shoots by wavelength dispersive X‐ray fluorescence spectrometry

Published: Apr 18, 2019

Author(s): Harjit Kaur Bajwa, Oinam Santosh, Ashwani Koul, M.S. Bisht, Chongtham Nirmala

Journal: X-Ray Spectrometry

Bamboo shoots are considered as healthy and nutritional food as they supply proteins, dietary fiber, phenols, phytosterols, vitamins, and minerals in considerable quantities and capture a novel place in the spectrum of plant foods. Besides nutrients and bioactive compounds, shoots also contain antinutrient (cyanogen) that needs to be removed by adequate processing prior to consumption. This study was undertaken to investigate the effects of fermentation, brine preservation, and boiling on...

Read More thumbnail image: Quantitative determination of macroelement and microelement content of fresh and processed bamboo shoots by wavelength dispersive Xray fluorescence spectrometry

Proteomes of Leaf‐Growing Zones in Rice Genotypes with Contrasting Drought Tolerance

Published: Apr 18, 2019

Author(s): Yunqi Wu, Mehdi Mirzaei, Dana Pascovici, Paul A. Haynes, Brian J. Atwell

Journal: PROTEOMICS

Abstract Plants require a distinctive cohort of enzymes to coordinate cell division and expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence, proteins in tissues sampled from leaves of a drought‐tolerant rice (IAC1131) are investigated to provide insights into the effect of soil drying on gene expression relative to the drought‐sensitive genotype Nipponbare. Shoot growth zones are dissected to estimate the proportion of dividing...

Read More thumbnail image: Proteomes of LeafGrowing Zones in Rice Genotypes with Contrasting Drought Tolerance

Radiative lifetime measurements of metastable levels in Kr3+ using electrostatic ion beam trap

Published: Apr 17, 2019

Author(s): M. Saito, H. Kubota, T. Majima, M. Imai, H. Tsuchida, Y. Haruyama

Journal: X-Ray Spectrometry

The radiative lifetimes of the metastable 4s24p3 2P3/2 and 2P1/2 levels in Kr3+ ions were measured using an electrostatic ion beam trap. The lifetimes were determined by measuring the number of photons emitted during the 2P3/2 → 4S3/2 and 2P1/2 → 4S3/2 forbidden transitions of the stored Kr3+ ions, as a function of the storage time. The obtained lifetime values for the 2P3/2 and 2P1/2 states were 23.3 ± 1.4 and 52.8 ± 3.8 ms, respectively. For the 2P3/2 state, this is the first experimental...

Read More thumbnail image: Radiative lifetime measurements of metastable levels in Kr3 using electrostatic ion beam trap

Towards experiments with highly charged ions at HESR

Published: Apr 17, 2019

Author(s): Rodolfo Sánchez, Angela Braeuning‐Demian, Jan Glorius, Siegbert Hagmann, Pierre‐Michel Hillenbrand, Anton Kalinin, Thomas Köhler, Yuri A. Litvinov, Nikolaos Petridis, Shahab Sanjari, Uwe Spillmann, Thomas Stöhlker

Journal: X-Ray Spectrometry

The atomic physics collaboration SPARC is a part of the APPA pillar at the future Facility for Antiproton and Ion Research. It aims at atomic‐physics research across virtually the full range of atomic matter. An emphasis of this contribution are the atomic physics experiments addressing the collision dynamics in strong electro‐magnetic fields as well as the fundamental interactions between electrons and heavy nuclei at the HESR. Here we give a short overview about the central instruments for...

Read More thumbnail image: Towards experiments with highly charged ions at HESR

Characterization of an Early Bronze Age pottery group from the upper Tigris valley (Turkey) by means of spectroscopic and microscopic techniques

Published: Apr 17, 2019

Author(s): Murat Bayazit, Gülriz Kozbe

Journal: X-Ray Spectrometry

In this archaeometric research potsherds from Kavuşan Höyük (a mound located in the upper Tigris valley) representing the Metallic Ware, a diagnostic ceramic group mostly found in north Mesopotamia was characterized using spectroscopic and microscopic techniques in order to differ the imitated ones, if exist, to reveal the production technologies and compare the results with the previous archaeometric works concerning Metallic Ware. For this purpose, optical microscopy, scanning electron...

Read More thumbnail image: Characterization of an Early Bronze Age pottery group from the upper Tigris valley Turkey by means of spectroscopic and microscopic techniques

Thermodynamic parameters for the complexation of water‐insoluble betulin derivatives with (2‐hydroxypropyl)‐γ‐cyclodextrin determined by phase‐solubility technique combined with capillary zone electrophoresis

Published: Apr 17, 2019

Author(s): Viktoria V. Sursyakova, Vladimir A. Levdansky, Anatoly I. Rubaylo

Journal: ELECTROPHORESIS

Abstract The complexation of betulinic and betulonic acids (BIA and BOA) (pentacyclic lupane triterpenoids) with (2‐hydroxypropyl)‐γ‐cyclodextrin (HP‐γ‐CD) was studied at different temperatures using the method combining phase‐solubility technique and CZE. In contrast to mobility shift ACE utilizing the electrophoretic mobility, in this approach, the peak areas are used. The apparent binding (stability, formation) constants are obtained by the Higuchi and Connors method from the linear segment...

Read More thumbnail image: Thermodynamic parameters for the complexation of waterinsoluble betulin derivatives with 2hydroxypropylcyclodextrin determined by phasesolubility technique combined with capillary zone electrophoresis

Nanoscaled porphyrinic metal–organic framework for photodynamic/photothermal therapy of tumor

Published: Apr 17, 2019

Author(s): Shengyan Wang, Weihua Chen, Chunhuan Jiang, Lehui Lu

Journal: ELECTROPHORESIS

Abstract Nanoagents achieving photodynamic therapy (PDT) and photothermal therapy (PTT) combination treatment with improved therapeutic effect are highly desirable. However, the incorporation of both PDT and PTT into a single nanoagent often requires multistep fabrication process. Herein, we report that photoactive porphyrin ligands have been successfully introduced into Zn‐TCPP structure to construct the nanoagents that possesses photodynamic performance and photothermal performance...

Read More thumbnail image: Nanoscaled porphyrinic metalorganic framework for photodynamicphotothermal therapy of tumor

Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertion/deletion polymorphisms

Published: Apr 17, 2019

Author(s): Xiao‐Ye Jin, Yuan‐Yuan Wei, Wei Cui, Chong Chen, Yu‐Xin Guo, Wen‐Qing Zhang, Bo‐Feng Zhu

Journal: ELECTROPHORESIS

Abstract Insertion/deletion (InDel) polymorphisms have been widely used in the fields of population genetics, genetic map constructions, and forensic investigations owing to the advantages of their low mutation rates, widespread distributions in the human genome, and small amplicon sizes. In order to provide more InDels with high discrimination power in Chinese populations, we selected and constructed one novel multiplex PCR‐InDel panel for forensic individual identification. Genetic...

Read More thumbnail image: Development of a novel multiplex polymerase chain reaction system for forensic individual identification using insertiondeletion polymorphisms

Quantitative analysis of microcystin variants by capillary electrophoresis mass spectrometry with dynamic pH barrage junction focusing

Published: Apr 17, 2019

Author(s): Ping Yan, Keke Zhang, Lingyu Wang, Wenjun Tong, David D. Y. Chen

Journal: ELECTROPHORESIS

Abstract Dynamic pH junction is an online focusing method in CE based on the electrophoretic mobility difference of analytes in the sample matrix and the background electrolyte. An advantage of this method over the conventional CE is that the sensitivity can be significantly improved. By injecting a long sample plug in the capillary and focusing the analytes at the pH boundary between the background electrolyte and sample matrix, the LOD can be improved by 10–100 folds. The dynamic pH junction...

Read More thumbnail image: Quantitative analysis of microcystin variants by capillary electrophoresis mass spectrometry with dynamic pH barrage junction focusing

An improved hybrid continuum‐atomistic four‐way coupled model for electrokinetics in nanofluidics

Published: Apr 17, 2019

Author(s): Saeed Jahromi, Ehsan Amani, Saeid Movahed

Journal: ELECTROPHORESIS

Abstract In this study, an efficient hybrid continuum‐atomistic method is proposed to study electrokinetic transport of aqueous solutions in nanofluidics. The aqueous phase is considered as a continuous phase containing immersed ion particles. The behavior of the system is then simulated through utilization of an improved hybrid continuum‐atomistic four‐way coupled approach, including the MultiPhase Particle‐In‐Cell method for the short‐ranged interaction between the ion particles, the...

Read More thumbnail image: An improved hybrid continuumatomistic fourway coupled model for electrokinetics in nanofluidics

Ethylene glycol: Evidence of glucuronidation in vivo shown by analysis of clinical toxicology samples

Published: Apr 17, 2019

Author(s): Daniel Sejer Pedersen, Patrick Bélanger, Mikael Frykman, Kirsten Andreasen, Danielle Goudreault, Henrik Pedersen, Peter Hindersson, Torben Breindahl

Journal: Drug Testing and Analysis

Abstract In the search for improved laboratory methods for the diagnosis of ethylene glycol poisoning, the in vivo formation of a glucuronide metabolite of ethylene glycol was hypothesized. Chemically pure standards of the β‐O‐glucuronide of ethylene glycol (EG‐GLUC) and a deuterated analog (d4‐EG‐GLUC) were synthesized. A high‐performance liquid chromatography and tandem mass spectrometry method for determination of EG‐GLUC in serum after ultrafiltration was validated. Inter‐assay precision...

Read More thumbnail image: Ethylene glycol Evidence of glucuronidation in vivo shown by analysis of clinical toxicology samples

Raman spectroscopy study of the La‐modified (Bi0.5Na0.5)0.92Ba0.08TiO3 lead‐free ceramic system

Published: Apr 17, 2019

Author(s): Y. Mendez‐González, A. Peláiz‐Barranco, A.L. Curcio, A.D. Rodrigues, J.D.S. Guerra

Journal: Journal of Raman Spectroscopy

Abstract A Raman spectroscopy study has been performed, at room temperature, on the (Bi0.5Na0.5)0.92Ba0.08‐3x/2LaxTiO3 lead‐free ceramic system (with x = 0, 1, 2, and 3 at.% La). The signature of the Raman spectra showed that the structural properties were noticeably affected by the La3+‐ions concentration, revealing the coexistence of two phases for the studied compositions. The structural properties showed the presence of both ferroelectric (P4mm) and antiferroelectric (P4bm) tetragonal...

Read More thumbnail image: Raman spectroscopy study of the Lamodified Bi0_5Na0_50_92Ba0_08TiO3 leadfree ceramic system

Areca alkaloids measured from buccal cells using DART‐MS serve as accurate biomarkers for areca nut chewing

Published: Apr 17, 2019

Author(s): Adrian A. Franke, Laura Biggs, Joanne Y. Yew, Jennifer F. Lai

Journal: Drug Testing and Analysis

Abstract Background Areca nut (AN) chewing is carcinogenic and biomarkers reflecting it are urgently needed to determine the effectiveness of emergent cessation programs. Buccal cells (BCs) may serve as an ideal matrix to measure such biomarkers; however, their utility for this purpose is unknown. Direct analysis in real time−mass spectrometry (DART−MS) is a sensitive technique that analyzes materials in the open air and requires minimal/no sample preparation. We utilized DART−MS to analyze...

Read More thumbnail image: Areca alkaloids measured from buccal cells using DARTMS serve as accurate biomarkers for areca nut chewing

Simultaneous quantification of propofol, ketamine and rocuronium in just 10 μL plasma using liquid chromatography coupled with quadrupole mass spectrometry and its pilot application to a pharmacokinetic study in rats

Published: Apr 17, 2019

Author(s): Teodora Shopova, Daniel Kiefer, Beate Wolf, Felix Maurer, Daniel I. Sessler, Thomas Volk, Tobias Fink, Sascha Kreuer

Journal: Biomedical Chromatography

Abstract The combination of propofol, ketamine and rocuronium can be used for anesthesia of ventilated rats. However, reliable pharmacokinetic models of these drugs have yet to be developed in rats, and consequently optimal infusion strategies are also unknown. Development of pharmacokinetic models requires repeated measurements of drug concentrations. In small animals, samples must be tiny to avoid excessing blood extraction. We therefore developed a drug assay system using high‐performance...

Read More thumbnail image: Simultaneous quantification of propofol ketamine and rocuronium in just 10 L plasma using liquid chromatography coupled with quadrupole mass spectrometry and its pilot application to a pharmacokinetic study in rats

A liquid chromatography–tandem mass spectrometry method to simultaneously determinate dichlorvos and phoxim in tobacco

Published: Apr 17, 2019

Author(s): Xinxian Qin, Xiaoshuang Luo, Ye Chen, Jiahua Han, Ji Zhang, Kankan Zhang, Deyu Hu

Journal: Biomedical Chromatography

Abstract A simple pretreatment method with liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed and validated to simultaneously determine dichlorvos and phoxim in tobacco and soil matrices. Satisfactory linearity (R2 ≥ 0.9991) of the method was obtained for both analytes. The limits of detection and limits of quantification for dichlorvos and phoxim in three matrices were 0.0015–0.006 and 0.005–0.02 mg/kg, respectively. Average recoveries were 78.24–92.21% for dichlorvos and...

Read More thumbnail image: A liquid chromatographytandem mass spectrometry method to simultaneously determinate dichlorvos and phoxim in tobacco
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